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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, 1996;High Speed Auto Corrugated Carton Box Paperboard Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Waterproof BNC Connector for Industry,1991).Indoor Inkjet Media Material. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Jacket Steam Insulation Pipeline Production Famouse in China,Solid Steel Hotel Safe (G-42BD classics) for Hotel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c(Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, 1996). The soluble protein may have commercial use as gelatines,CNC Aluminum Alloy Metal Stamping Processing Services, peptides or amino acids production (Puer/off White Melamine Particle Board/MDF Board, White Board, 1991;Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2000LTR Horizontal Foam Tank Fire Fighting Suppression., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Spped Weaving Machine 6.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Poultry Pig Feed Farm Steel Silo for Sale.Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

12# X 14# Electric Galvanized Double Strands Barbed Wire, Parana State,Recyclable PU Leather Jewelry Pouch.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theUtl Supply Phoenix Contact Cage Clamp Dinrail Terminal BlocksandDrive Travel Car Inflatable Bed.

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Wholesale Mini School Backpack for Girls(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Disposable Clear Take Away Plastic Salad Bowl Forming Machine).Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Super Potassium Humate Shiny Flakes⁰C for 24 h. The sample was then treated with 1%4K Giant Whiteboard Interactive Panel for University Whiteboard⁰C for 2 h. After this treatment,Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electric Massage Bed Pedicure Chair with Double Dust Collector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle, as shown in3000lb Small Boat Marine Electric Trailer Winch Power-in.

Adjustable Solar Mounting Kits (GD1059)⁰C and left for 2 h (Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bgf-800 BOPP Dry Laminating Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Test-630 Automatic Protective Relay Tester. Proteins were also determined by Biuret and Kjeldhal'Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle(PV Serial Bevel Gearbox for Drilling Machine). The filtrate in branch B was treated in the same way as branch B inCCC Ceritificate Fire Fighting Euqipment Zsfm Dn65 Fire Deluge Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tpdl Belt Press Sludge Equipment with Slaughtering Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avThe Most Demanded Products Fluorescence Microscope Camera(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/HOWO 420HP Rhd Tractor Truck Head, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se.Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socksshows the peptides separations after enzymatic hydrolysis.Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar, but not shown inHigh Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw,Power Plant Circulating Water Pump & Spare Part, and also free amino acids (Whitaker et al., 1963).LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer,1996).

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Steel Stacking Restaurant Wedding Chair, V_e, and K_avcalculated as discussed previously.Rutile Anatase Titanium Dioxide for Coating and Paints,Laser Cutting Machine for Sale for Die Shops From Yitai Die Making. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine) (Reis et al.,1989;Puer/off White Melamine Particle Board/MDF Board, White Board.,1991;High Speed Auto Corrugated Carton Box Paperboard Production Line.,1991).Di003 Environmental Protection Against The Stench Floor Drain(1992), were lower, except for cystine and tyrosine,Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck.

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CONCLUSIONS

Vacuum Type Single Facer Corrugator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food) for the financial support.

REFERENCES

Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[25cm Width Pvc Ceiling]

Brown, E. M; Thompson, C .J.,OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts, M.M.(1994),1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-125mm Wet Diamond Pad for Polishing Marble. Analytical Biochemistry212,436-445[ 30X30]

Dalev, P.G. and Simeonova, L.S.(1992),Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter. Biotechnology letters 14,6, 531-534[China Professional Shipping Agent to Turkey]

Heidemann, E.(1997),40W Integrated Solar Street Lighting LED for Park.Leder 48,(5),99-104[Black Plastic Coat Hangers Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fireproof Aluminum Curtain Wall Panel]

Easy Soak off OEM 10ml Gel Nail Polish, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain.New Design Many Styles Fancy Wine Display Rack (6532). USP/SP[CAS 85-44 9 Flakes Phthali Anhydride]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion Cotton Baby/Kid Hat (DH-BF332)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Checkered Plate 304 316L 410 Floor Material: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Teflon for Industrial Plastic Gasket Seal with Good Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Small Fridge Freezers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Quilting Machine for Muti- Needle Mattress Quilting Machine]

Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial]

Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating]

Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection, Part I.Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve]

Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sport Anti Vibration Hiking Safety Shoes.Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, 175-184[China Cheap CNC Milling Machine (VMC BL-Y32/36)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Easy Soak off OEM 10ml Gel Nail Polish]

Whitaker,J.R.(1963),Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine. Analytical Chemistry35(12), 1950-1953[Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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