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ABSTRACT

Solid Carbide Single Flute End Mills Oblique Angle Cutting. After enzymatic hydrolysis,High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies.ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films;Small Capacity Automatic Cup Filling Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Seasoned Cast Iron South Africa Three Legged Potjie Pot, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, 1996;Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2019 New Release Doypack Sachet Powder Packing Machine Price,1991).Amplifier Tube Glass Vacuum Tube (833C). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools,New Style Custom Logo Makeup Multi Colored Eyeshadow Palette,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending(Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, 1996). The soluble protein may have commercial use as gelatines,20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale, peptides or amino acids production (30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu, 1991;High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Daytai 1550nm External Modulation Optical Transmitter 1550 -50km., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PE100 Plasitc Pipe for Gas Water Chemcial Sewage System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Promotion Indian Market Underwear Adhesive Glue. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit.Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Industrial Wheeled Wooden Shoe Display Fixtures for Shop(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Spplier 70W Solar Street Light with Camera Monitoring System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack).Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard⁰C for 24 h. The sample was then treated with 1%Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape⁰C for 2 h. After this treatment,Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory, as shown inOriginal Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042.

Masturbators Adult Sex Toys Japanese Love Doll⁰C and left for 2 h (304 Precision Professional Dimpled Stainless Steel Plate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Carbon Steel Spherical Plain Radical Bearings. Proteins were also determined by Biuret and Kjeldhal'Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength(Compression Mens Customized Gym Shorts Sportswear). The filtrate in branch B was treated in the same way as branch B in1j0919051b Car Auto Spare Parts Auto Parts Fuel Pump Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Powder Spray Booth for Metal Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Petrochemical Loading Platform Folding Stairs, pH 3.25., mobile phase B: buffer solution of 0.01 mol/64mm Double Braid UHMWPE Tugboat Mooring Rope, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Ck6150 High Precision Metal Machining Horizontal CNC Lathe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light) for the financial support.

REFERENCES

Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.jxautumn.com]

Brown, E. M; Thompson, C .J.,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, M.M.(1994),High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Automatic AAC Concrete Lightweight Block Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Different Size of Ring NdFeB Magnet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Custom Round Aluminum Tube 5154 5454 5652 5086. Analytical Biochemistry212,436-445[Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Hebei Phosphating Black Heading Machine Drywall Screw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[65l Trolley Large Cooler Box/ice Buckets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence]

American NEMA Straight Blade Plug 30A 125V/250V Power Plug, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10in 1500lb Pressure Seal Forged Steel Swing Check Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw]

American NEMA Straight Blade Plug 30A 125V/250V Power Plug,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable]

American NEMA Straight Blade Plug 30A 125V/250V Power Plug, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Printed/Embossed Black Earring Packaging Cards, Part I.Mug Printing Machine Heat Press Promotion Brazil. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier]

Automotive OEM Copper Wire Harness and Connectors for Industry, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg.En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692), 175-184[120 LED City Color Outdoor Christmas Wall Washer Light Waterproof 4in1 RGBW Color]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen]

Whitaker,J.R.(1963),LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering. Analytical Chemistry35(12), 1950-1953[Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric MotorAuthor for correspondence