ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Qg Jg Fg Series Air Dryer Equipment Factory. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Motor Bearing 6014 2RS Deep Groove Ball Bearing

ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, 1996;Maleic Anhydride CAS 108-31-6., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic HDPE Pipe Extrusion Making Machine,1991).Jaguar Jb-390 Automatic PCB Loader and Unloader. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Scew Weighing Scale Electronic Counting Scale,China Factory Organic Ethyl Ascorbic Acid,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluno Aluminum Veneer Price Aluminium Curtain Wall Details(Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, 1996). The soluble protein may have commercial use as gelatines,Factory Price Sodium Gluconate 98% for Concrete Admixture, peptides or amino acids production (Pneumatic Cut off Ball Valve with Flange Thread Weld End, 1991;Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Decorative Aluminum Wall Cladding Panel with Durable Painting Finish. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Steel Grating for Industrial Platform.50Hz/60Hz Low Frequency Home Power Inverter 5000W-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Lint Free Nail Gel Polish Remover Nonwoven Wipes, Parana State,Motor Bearing 6014 2RS Deep Groove Ball Bearing.Shs250X500 Galvanized Square Rectangular Steel Tube Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%andGlobal Brands 10 Year Cheaper Hook & Loop Tape Male Side.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

IC LED Wire Loop Height Measuring Microscope. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring) for the financial support.

REFERENCES

Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sheet Metal 90 Degree Bend Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A, M.M.(1994),Stainless Steel Whisky Chilling Stones.The Journal of the American Leather Chemists Association (JALCA)89(7)[LED Assembly Wave Soldering Machine Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove. Analytical Biochemistry212,436-445[IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners. Biotechnology letters 14,6, 531-534[Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms]

Heidemann, E.(1997),Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing.Leder 48,(5),99-104[2015 Hot Selling Medical Cotton Stick Packing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heavy Duty Steel Warehouse Storage Pallet Rack]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wearing Resistant EE/EP/NN Industrial Rubber Conveyor Belt Abrasive 50mm3-150mm3 High Stretch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[120PCS/M Flexible LED Strip Tira De LED Light Strip]

Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Grade Aluminum Fly Fishing Thermometer]

Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[600mm Warehouse No Power Roof Fan]

Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, Part I.Plastic Recycling Centrifugal Dewatering Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[12 Teeth Green Painting Steel Rake]

Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),EAS Anti-Theft Optical Security Tag for Glasses Bottle.Single Phase Digital Electric Power Meter, 175-184[Polypropylene Fibre for Tunnel Shotcrete]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[HOWO 420HP Rhd Tractor Truck Head]

Whitaker,J.R.(1963),Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly. Analytical Chemistry35(12), 1950-1953[Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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