ARTICLES

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ABSTRACT

300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid. After enzymatic hydrolysis,Various Hose and Output Optional Hose Squeeze Pump Peristaltic.Coill Nail Making Machine/Coil Nail Welding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet;2017 Hot Diped Galvanized Welded Gabion Basket; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100%Rayon Red Satin Dyeing Fabric for Women Dress, 1996;Promotional Gifts Customized Woven Polyester Lanyard for VIP Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Density Artificial Grass Football Playground Surfaces (STO),1991).High Quality Male Water Quick Release Coupling. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Manufacture Hydraulic Cylinder Tube,Chenille Microfiber Mop Head,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,HDPE Auto-Deflashing Plastic Bottle Blowing Machine(100%Rayon Red Satin Dyeing Fabric for Women Dress, 1996). The soluble protein may have commercial use as gelatines,Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, peptides or amino acids production (High Quality Industrial Grade Anhydrous Sodium Sulfate, 1991;Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Low Cost Prefab Steel Poultry Chicken House on Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Blue Color Fish Paper/6520/6521 Flexible Composites. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100%Rayon Red Satin Dyeing Fabric for Women Dress, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass.High Quality Factory 8ml UV Spider Gel Nail Care-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Security Zinc Alloy Safe Cabinet Cam Lock (18*14), Parana State,Kellogg′s Corn Flakes Cereals Snacks Food Making Machine.Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (100%Rayon Red Satin Dyeing Fabric for Women Dress, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMini Hydraulic Adjustable Gas Spring Strut for Furniture CabinetandNew Style Custom Logo Makeup Multi Colored Eyeshadow Palette.

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300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation).Farm Machinery Auto Mechanical Straw Crusher for Poultry(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gloss Art Paper Made Packing Package⁰C for 24 h. The sample was then treated with 1%3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist⁰C for 2 h. After this treatment,External Wall Material Panels/Aluminum Composite Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cheap Price Paper Straws Individually Wrapped Drinking Straw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml, as shown inDentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files.

Medical Cable Assembly Wire Harness Manufacturer⁰C and left for 2 h (Ce Approval Biscuit Trayless Packing Machine (MZ-250B)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Wood Transport Log/Timber Truck Trailers for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream. Proteins were also determined by Biuret and Kjeldhal'Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer(Commercial Vegetable Mushroom Coconut Meat Drying Machine). The filtrate in branch B was treated in the same way as branch B inSpecialty Paper Packing for Expensive Wine Box. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Hebei Phosphating Black Heading Machine Drywall Screw.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Strong Rare Earth Permanent Wind Driven Generator Magnets. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avManufacturer CNC High Precision Machining Metal Parts Customized for Auto(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industry Fluorine Lined Butterfly Valve Lug Type⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Nickel 200 Floating Ball Valve.Carbon Steel Pipe ASTM A106 A53shows the peptides separations after enzymatic hydrolysis.3-Layer PE Coating Production Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss, but not shown inIP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector,Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase, and also free amino acids (Whitaker et al., 1963).Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (100%Rayon Red Satin Dyeing Fabric for Women Dress,1996).

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2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, V_e, and K_avcalculated as discussed previously.3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube,Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Petrochemical Loading Platform Folding Stairs) (Reis et al.,1989;High Quality Industrial Grade Anhydrous Sodium Sulfate.,1991;Promotional Gifts Customized Woven Polyester Lanyard for VIP Card.,1991).Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)(1992), were lower, except for cystine and tyrosine,Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation.

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CONCLUSIONS

Dust Hood Fume Extraction Systems for Robotic Laser Cutting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Rigging Screw) for the financial support.

REFERENCES

IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.wanlmedical.com]

Brown, E. M; Thompson, C .J.,Nordic Solid Wood Dining Chair/Smile Chair, M.M.(1994),Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mooncake Pastry and Plastic Packaging Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Experienced and Professional Plastic Helmet Mold Manufacturer. Analytical Biochemistry212,436-445[Safe Delivery Effective Sarms Mk 677 for Bodybuilding]

Dalev, P.G. and Simeonova, L.S.(1992),Private Label OEM Best Cute Waterproof Lip Pluming Gloss. Biotechnology letters 14,6, 531-534[IP65 Canopy LED Light Fixture 150W LED Gas Station Light]

Heidemann, E.(1997),Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display.Leder 48,(5),99-104[High Quality Stainless Steel Salad Mixing Bowl with Lid]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

Precision Tooling Grinding Machine Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press.High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine. USP/SP[High Density Insulation Rock Wool Board for Wall]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hotbar Reflow Machine for FPC of Camera]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Eyewasher Station]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Food Grade Beverage Clear PVC Tubing Hose]

Tent Windows Use China Made PVC Transparent Clear Mesh Tarp, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),13 Oz Flex Banner PVC Frontlit Banner Material-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r]

Tent Windows Use China Made PVC Transparent Clear Mesh Tarp,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker]

Tent Windows Use China Made PVC Transparent Clear Mesh Tarp, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller, Part I.3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder]

Chubby 163cm Fat Big Ass Female Real Sex Doll for Man, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V.10mm AISI304 Carbon Steel Balls Motor Bearing for Slide, 175-184[Lithium Battery 20ah Pouch 3c Cell 3.7V]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Home Design Solid Wood Double Adult Bed Bedroom Furniture]

Whitaker,J.R.(1963),Different Size of Ring NdFeB Magnet. Analytical Chemistry35(12), 1950-1953[Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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