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ABSTRACT

Maleic Anhydride CAS 108-31-6. After enzymatic hydrolysis,Plastic HDPE Pipe Extrusion Making Machine.Jaguar Jb-390 Automatic PCB Loader and Unloader. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Scew Weighing Scale Electronic Counting Scale;China Factory Organic Ethyl Ascorbic Acid; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Price Sodium Gluconate 98% for Concrete Admixture, 1996;Pneumatic Cut off Ball Valve with Flange Thread Weld End., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine,1991).Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor,Decorative Aluminum Wall Cladding Panel with Durable Painting Finish,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Steel Grating for Industrial Platform(Factory Price Sodium Gluconate 98% for Concrete Admixture, 1996). The soluble protein may have commercial use as gelatines,50Hz/60Hz Low Frequency Home Power Inverter 5000W, peptides or amino acids production (ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line, 1991;Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Price Sodium Gluconate 98% for Concrete Admixture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lint Free Nail Gel Polish Remover Nonwoven Wipes.Shs250X500 Galvanized Square Rectangular Steel Tube Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Water Filter Cartridge PP Sediment Filter Cartridge(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt).Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Disposable Skin Stapler (CSPF-35W)⁰C for 24 h. The sample was then treated with 1%Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)⁰C for 2 h. After this treatment,New Customized Mug Heat Press Machine Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory, as shown inEmbossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal.

Shengya Steel Concrete Electric Pole Mould⁰C and left for 2 h (Hot Double Cereal Airtight Food Dispenser). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable. Proteins were also determined by Biuret and Kjeldhal'Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles(High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line). The filtrate in branch B was treated in the same way as branch B inBrass Cabinet Door Ball Catch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Manufacturer Garbage Incinerator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China water meter factory water flow meter vane wheel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4 Heads High Speed Professional Paper Cardboard Laser Cutter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Gas Calcined Anthracite Carburizing Agent, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Good Quality Custom Car Power Window Cable for Ford Car. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die) for the financial support.

REFERENCES

Solar Panel PV System Mc4 Circular Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Top Grade 99.5% Nh4cl Ammonium Chloride CAS 12125-02-9]

Brown, E. M; Thompson, C .J.,1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, M.M.(1994),Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Roots Vacuum]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wire Mesh Welding Size 10mm X 10mm]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting. Analytical Biochemistry212,436-445[Promotional Gifts Customized Woven Polyester Lanyard for VIP Card]

Dalev, P.G. and Simeonova, L.S.(1992),Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall. Biotechnology letters 14,6, 531-534[PA Flexible Plastic Corrugated Loom Tubing]

Heidemann, E.(1997),Plastic Recycling Centrifugal Dewatering Machine.Leder 48,(5),99-104[Amino Acids Filtration]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Abstract Light and Shadow Metal Oil Painting for Deco]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Single Phase Digital Electric Power Meter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Field Fence 84]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cute Cartoon Coin Purse Children Zipper Purse]

Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2W 100lm LED Solar Lawn Light for Garden or Yard Lighting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Construction Material Hoist Rack and Pinion]

Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb]

Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, Part I.28mm China High Quality Supplier Screw Cannon Cap for Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sc Fiber Optic Fast Connector]

Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure.Nonwovenface Mask Production Line, 175-184[Biodegradable Eco-Friendly Food Dessert Serving Bamboo Round Plates]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Carbon Steel Spherical Plain Radical Bearings]

Whitaker,J.R.(1963),Wholesale Professional Indoor&Outdoor Trampoline Park. Analytical Chemistry35(12), 1950-1953[ Carbon Steel 20g Gr. B Steam Cooling Fin Tube]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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