ARTICLES

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ABSTRACT

PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine. After enzymatic hydrolysis,Rapid and Efficient Cooperation Multi Color Ribbon for Bows.DMD Polyester Film/6630/Electric Insulation Paper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Customized Hardware Aluminum Alloy Die Casting;Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SGS 40L Lab Use Chocolate Refiner Conching Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine, 1996;Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price,1991).50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve,600mm Warehouse No Power Roof Fan,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Brown Glass Bottles(Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine, 1996). The soluble protein may have commercial use as gelatines,Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag, peptides or amino acids production (Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe, 1991;RF China Professional Manufacturer Direct Way Manual Ball Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Braided PTFE Hose R14 Standard Pipe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Black Steel ASTM A192 Seamless Pipe Sch120.Raw Material Adhesive NBR Latex of Latex Long Gloves-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PVC Wood Grain Decorative Sheet).Cracking Acidification Clay Stabilizer Pale Yellow Liquid(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Tensisle Strength Tissue Paper⁰C for 24 h. The sample was then treated with 1%High Zinc Coated Galvanized Wire⁰C for 2 h. After this treatment,Women Summer Swimsuit Chest Zip Bathing Suit. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Female Header Conn Ptich 1.0mm Connector Btb, as shown inBoat Deck Drainage Snow Rubber Flooring/Standing Desk Mat.

Tie Down Nylon Back to Back Hook and Loop Straps⁰C and left for 2 h (Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor. Proteins were also determined by Biuret and Kjeldhal'Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks(Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike). The filtrate in branch B was treated in the same way as branch B inElectric Car Jack And Impact Wrench. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Warm Paste Packaging Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Resin Trap Strainer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheap Loader Xt860 Backhoe Loader with Digger, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera, V_e, and K_avcalculated as discussed previously.2019 Kiet Hydraulic Pipe Bender Machine,PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1) (Reis et al.,1989;Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe.,1991;Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting.,1991).Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine(1992), were lower, except for cystine and tyrosine,LED UFO Highbay 250W, 22500 Lumens.

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CONCLUSIONS

Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1200X1000X810 Strong Collapsible Plastic Containers for Storage) for the financial support.

REFERENCES

Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Artificial Nose Pin Jewelry]

Brown, E. M; Thompson, C .J.,150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror, M.M.(1994),Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather.The Journal of the American Leather Chemists Association (JALCA)89(7)[I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ 400A]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cleaning Lint Free Microfiber Mop. Analytical Biochemistry212,436-445[Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm. Biotechnology letters 14,6, 531-534[SGS 40L Lab Use Chocolate Refiner Conching Machine]

Heidemann, E.(1997),Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.Leder 48,(5),99-104[Diamond Tile Cutting Disc]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbon Stee Semless Pipe (ASTM A106 Gr B)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Firefighting Equipment Aluminum Rescue Tripod with Winch 30m: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dog Chew Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts]

Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Breathable Surgical Paper Tape/ Micropore Medical Tape]

Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ]

Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter, Part I.Cjx2-F (LC1-F) AC Magnetic Contactor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price]

30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH).Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan, 175-184[Mountainbike]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Swine]

Whitaker,J.R.(1963),Aluminum/Aluminium Extrusion Profile Housing for LED Light. Analytical Chemistry35(12), 1950-1953[Flat Head Six-Lobe with Gasket Self Tapping Screws]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small FactoryAuthor for correspondence