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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, 1996;500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (RFID Blocking Leather Slim Zipper Credit Card Holder Wallet,1991).2017 New Design Vinyl 3D Stone Wallpaper Factory in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Small Paper Gift Box with Magnet Closure for E-Cigarette,Agricultural Mini Tiller Farm Wheel 62cc Cultivator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price(Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, 1996). The soluble protein may have commercial use as gelatines,Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns, peptides or amino acids production (High Efficient Online Hot Glue Dispensing Machine for Electronic Products, 1991;High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automotive Sheet Metal Stamping Car Auto Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable.2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1080W Most Powerful LED Flood Light Fixtures(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Glass Water Jet Cutting Machine (Skwj-4018A)).OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor⁰C for 24 h. The sample was then treated with 1%High Quality Bucket Segment Bolt 6V0937⁰C for 2 h. After this treatment,Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Server Rack Network Data Cabinet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera, as shown inAutomatic CNC Centerless Lathe for Peeling Steel Round Bar.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPeptides Powder Ghrp 2 for Muscle Building with Super Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.EPDM, PVC Car or Door Rubber Sealing Strip⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Black Color Tube Eyelet Lift Gas Spring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Safety Edge Sensor for Agv Automated Guided Vehicle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Foteck SSR 40da Solid State Power SSR Relay. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine) for the financial support.

REFERENCES

2015 New Square Folding Ajustable Wall LED Bathroom Mirror, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[KIA-1kw Handheld Household Portable Induction Heater]

Brown, E. M; Thompson, C .J.,Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle, M.M.(1994),13.8cm Reed Diffuser Clear Glass Bottle for Aroma.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Efficiency Air Cooled Air Source Water Chiller Heat Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[360 Degree Reciprocating Car Wash Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Metal Cutting Machine CNC Plasma Cutter. Analytical Biochemistry212,436-445[High Quality Endoscopic Surgical Adult Disposable Bite Block]

Dalev, P.G. and Simeonova, L.S.(1992),Medical Suction Connecting Tube with Yankauer Suction Handle. Biotechnology letters 14,6, 531-534[Insulation Anti Earthquake Exterior Wall Foam Board]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ycom USB Charger and Data Cable with Aluminum for Type C]

Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant.Self-Propelled Wheel Farm Using 3 Rows Corn Harvester. USP/SP[5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Right Angle Worm Gearbox for Food and Beverage and Packaging Machines: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Single Sphere Rubber Expansion Joint]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fertilizer Granulation Plant]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[White Pattern Acrylic Sheet for Decoration Produced by Senpu]

Manufacturer High Strength Carbon Steel Heavy Hex Nut, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Spplier 70W Solar Street Light with Camera Monitoring System]

Manufacturer High Strength Carbon Steel Heavy Hex Nut,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks]

Manufacturer High Strength Carbon Steel Heavy Hex Nut, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Seamless Coil Tube ASTM A249/A269, Part I.The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Army Style 100% Polyester Fiber Mens Polo Shirt]

Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hardware Rigging Forged Red Weldless Pear Shaped Link.Hot Water Pipe Insulation Steam, 175-184[Honeyson Wholesales Electronic Password Hotel Hidden Safe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft]

Whitaker,J.R.(1963),Economy Combo Heat Press for Starter. Analytical Chemistry35(12), 1950-1953[Quality Assurance Advanced Design Microfiber Filling Washable Pillow]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Qingdao Us Type Load Binder Lever Chain TensionerAuthor for correspondence