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ABSTRACT

Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger. After enzymatic hydrolysis,Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure.Nonwovenface Mask Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.HVAC Ventilation Supply Air Return Grille Air Vent, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door, 1996;65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Output Vulcanizing Press Machine,1991).CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W,Transmission Ultrasound Gel /20ml, 250ml, 5L,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire(High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door, 1996). The soluble protein may have commercial use as gelatines,Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence, peptides or amino acids production (Food Grade Ivory Paper Cupcake Boxes, 1991;Slide Switch - DC Power Jack RoHS, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2018 New Style High Quality Enamel Bowl with Lid.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Roots Vacuum, Vacuum Pump, Pulp Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes.Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Solar DC Water Pump, Solar Submersible Pumping System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Quality Banded Type Tee).55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow⁰C for 24 h. The sample was then treated with 1%Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V⁰C for 2 h. After this treatment,China Manufacturer Mini Cultivator for Small or Garden Farming. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Art-Screen for Lobby, as shown inNatural OEM Private Label Customized Skin Care Face Serum.

Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch⁰C and left for 2 h (Black Plastic Handle Stainless Steel Kitchen Gadget). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat. Proteins were also determined by Biuret and Kjeldhal'Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts(Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply). The filtrate in branch B was treated in the same way as branch B inwww.sajoo-switch.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/120PCS/M Flexible LED Strip Tira De LED Light Strip, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT) for the financial support.

REFERENCES

Twisted Annealed Wire for Binding, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ad26b22BS Shorter Type LED Indicator Light with CB/Ce/CCC Certificated]

Brown, E. M; Thompson, C .J.,Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, M.M.(1994),Wholesale Fashion Jewellery Lady Eardrop Earrings for Women.The Journal of the American Leather Chemists Association (JALCA)89(7)[Laser Cutting Machine for Sale for Die Shops From Yitai Die Making]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640. Analytical Biochemistry212,436-445[Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads]

Dalev, P.G. and Simeonova, L.S.(1992),Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings. Biotechnology letters 14,6, 531-534[From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die]

Heidemann, E.(1997),Full Automatic AAC Concrete Lightweight Block Making Machine.Leder 48,(5),99-104[ASME B 16.5 Flange Cryogenic Floating Ball Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Expanded Mesh]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Watermark Approval Brass Press Equal Fitting Male Tee]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC / Galvanized Chain Link Metal Security Fence for Farm / Garden]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Medical Suction Connecting Tube with Yankauer Suction Handle]

High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger]

High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Price Aluminium Sulphate for Water Treatment]

High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Low Volume Production Aluminum CNC Machining Auto Parts, Part I.Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle]

Whitaker,J.R.(1963),Cable Length 11 Gun Safety Padlock. Analytical Chemistry35(12), 1950-1953[White Crystal Powder Betaine Anhydrous CAS#107-43-7]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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