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ABSTRACT

White and Green Board, School Writing Board, School Furniture (GT-76C). After enzymatic hydrolysis,Current Precise LED Digital Ammeter.Automatic Drinking Mineral Water Filling Line/Water Bottling Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Disposable Sugarcane Pulp Paper Bowl 500ml, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Flat Roof Insulation Board Heat Insulation Board, 1996;2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Female Threaded Tee T Shape Pipe Fittings,1991).Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine,Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste(Flat Roof Insulation Board Heat Insulation Board, 1996). The soluble protein may have commercial use as gelatines,Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools, peptides or amino acids production (8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, 1991;Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Natural OEM Private Label Customized Skin Care Face Serum., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Screw Press Dewatering Machine for Cassava Residue. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Flat Roof Insulation Board Heat Insulation Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Outdoor Indoor Football Soccer Goal Post Net for Kids.Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring).Single Block Vertical Wire Drawing Machine for Screw and Bolt Making(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Professional Shipping Agent to Turkey⁰C for 24 h. The sample was then treated with 1%White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment⁰C for 2 h. After this treatment,Crusher for Animal Waste/ Chicken Manure. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Threaded Locking Circular Connectors M16 680, as shown inoff Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTitanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waterproof LED Flash Shoe Light Crystal Epoxy Resin, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Shopping Paper Bag for Clothing Package, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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14 Heads Double Door Multihead Weigher.Industrial Threaded Plastic Connector Female Electrical Plugshows the peptides separations after enzymatic hydrolysis.Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAdjustable High Voltage Operating Rod Stick Telescopic Hot Stick, but not shown inWaterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring,White Marble, Carrara White, Bianco Carrara Marble for Sale, and also free amino acids (Whitaker et al., 1963).Upper Limbs Machine/Spare Parts for Fitness Equipment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Flat Roof Insulation Board Heat Insulation Board,1996).

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CONCLUSIONS

Industrial Sausage Meat Smoker Oven/Smokerhouse Oven. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Density Insulation Rock Wool Board for Wall) for the financial support.

REFERENCES

Men′s High Quality Mercerised Cotton Business Dress Socks, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Iron Church Chair Theater Seat]

Brown, E. M; Thompson, C .J.,Livining Room Rugs Geometric Patterns Carpets 6′*9′, M.M.(1994),Lamella Clarifier for Wastewater Treatment Plant.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Soft PVC Granule Used for Refrigerator Door Gasket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Products Suppliers Camlock Coupling Quick Couplings Female]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine. Analytical Biochemistry212,436-445[2019 New Release Doypack Sachet Powder Packing Machine Price]

Dalev, P.G. and Simeonova, L.S.(1992),Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen. Biotechnology letters 14,6, 531-534[PVC Corrugated or Smooth Surface Helix Suction Water Hose]

Heidemann, E.(1997),Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S).Leder 48,(5),99-104[Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-E6000 Handheld Three Phase Power Quality and Energy Analyzer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ASA-UPVC Anti-Corrosive Composite Roof Tile]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[40W Integrated Solar Street Lighting LED for Park]

Qg Jg Fg Series Air Dryer Equipment Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Motor Bearing 6014 2RS Deep Groove Ball Bearing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FKM Rubber Gasket/Rubber O Rings in China]

Qg Jg Fg Series Air Dryer Equipment Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Safety Edge Sensor for Agv Automated Guided Vehicle]

Qg Jg Fg Series Air Dryer Equipment Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line, Part I.Pet Short Fiber Non Woven Geotextile for Landfill Filtration. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine]

Pre Painted Steel Coil and Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd.Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor, 175-184[90% 99% Alumina Ceramic Raschig Rings From Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT)]

Whitaker,J.R.(1963),8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8). Analytical Chemistry35(12), 1950-1953[Luxury Jewelry White Gold Plated Engagement Diamond Ring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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