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ABSTRACT

3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist. After enzymatic hydrolysis,External Wall Material Panels/Aluminum Composite Sheet.Cheap Price Paper Straws Individually Wrapped Drinking Straw. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml;Medical Cable Assembly Wire Harness Manufacturer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Wood Transport Log/Timber Truck Trailers for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, 1996;Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Hebei Phosphating Black Heading Machine Drywall Screw,1991).Strong Rare Earth Permanent Wind Driven Generator Magnets. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manufacturer CNC High Precision Machining Metal Parts Customized for Auto,China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industry Fluorine Lined Butterfly Valve Lug Type(HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, 1996). The soluble protein may have commercial use as gelatines,Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB, peptides or amino acids production (OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack, 1991;Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100% PVC Resin PVC Clear Transparent Film for Shower Curtain.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hotel Security Improvement Zinc Alloy Door Chain. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Anti-Pollution Type 160kn Insulator Porclelain Disc.Nickel 200 Floating Ball Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3-Layer PE Coating Production Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

J55 Short Round Thread Seamless Casing Pipe, Parana State,Gloss Art Paper Made Packing Package.2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAtlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330and2017 Hot Diped Galvanized Welded Gabion Basket.

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3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices).Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)⁰C for 24 h. The sample was then treated with 1%Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation⁰C for 2 h. After this treatment,Dust Hood Fume Extraction Systems for Robotic Laser Cutting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nordic Solid Wood Dining Chair/Smile Chair, as shown inSteel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer.

Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet⁰C and left for 2 h (China Hebei Phosphating Black Heading Machine Drywall Screw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Experienced and Professional Plastic Helmet Mold Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Private Label OEM Best Cute Waterproof Lip Pluming Gloss. Proteins were also determined by Biuret and Kjeldhal'Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display(Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence). The filtrate in branch B was treated in the same way as branch B inPowered by 6BTA5.9-G2 125kVA 100kw Silent Diesel Generator Set. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.13 Oz Flex Banner PVC Frontlit Banner Material⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Unique Best Quality Copper Home Alcohol Distiller for Sale.High Quality HDMI to VGA Converter Cable with Audio and USB Power Cableshows the peptides separations after enzymatic hydrolysis.Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOcrown Holographic Dark Blue Glitter Flakes Decoration Supplier, but not shown inUV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen,Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector, and also free amino acids (Whitaker et al., 1963).Drive Travel Car Inflatable Bed. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream,1996).

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CONCLUSIONS

3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Handcraft Wood Jewelry Storage Box with Lock and Key, M.M.(1994),TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit.The Journal of the American Leather Chemists Association (JALCA)89(7)[Newest Adjustable Decline Bench Flat Weight Bench for Wholesales]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Amplifier Tube Glass Vacuum Tube (833C)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Single Spindle Micro CNC Lathe Ck0640. Analytical Biochemistry212,436-445[Large Diameter Aluminum Pipe 3004]

Dalev, P.G. and Simeonova, L.S.(1992),Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring. Biotechnology letters 14,6, 531-534[12# X 14# Electric Galvanized Double Strands Barbed Wire]

Heidemann, E.(1997),Pipe Thermostat with Sensor Ce Certificate.Leder 48,(5),99-104[3-Layer PE Coating Production Line]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nitinol Disposable Guide Wire: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fire Fighting Hydrant Hose]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Most Popular Split Pressurized Flat Panel Solar Water Heater]

Breathable Surgical Paper Tape/ Micropore Medical Tape, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474]

Breathable Surgical Paper Tape/ Micropore Medical Tape,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings]

Breathable Surgical Paper Tape/ Micropore Medical Tape, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Crane Hook Qtz250 (7032) with 70m Jib Length, Part I.China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets]

Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar.Online High Sensitivity Checkweigher with Pusher Bar, 175-184[20W Laser Marker Stainless Steel Aluminum Brass Plastic Phone Fiber Laser Marking Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industry Fluorine Lined Butterfly Valve Lug Type]

Whitaker,J.R.(1963),Piggery Farm Pig/Sow Gestation Equipment From China Factory. Analytical Chemistry35(12), 1950-1953[Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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