ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Black Powder Coated Steel Sheet Metal Housing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring

ABSTRACT

Hot Sell 100% Seal Hexagon Glass Jam Jar. After enzymatic hydrolysis,Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping.High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape;Heavy Caliber Copper Rectangular Steel Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2mm White Coated Paper Cake Boards Jordan, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, 1996;Luxious Hotel Passenger Elevator, Titanium Elevator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Yuchai 400kw Diesel Generator Set,1991).Black Plastic GRP FRP PE Pipe Fitting for Water Supply. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2194230012 for Mercedes Benz Auto Spare Parts Brake Disc,Precision Casting Factory Foundry OEM Ductile Iron Sand Casting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Electrical Motors Small for Home Appliance(Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, 1996). The soluble protein may have commercial use as gelatines,PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, peptides or amino acids production (Bending Shrapnel, 1991;12V Car Cigarette Lighter Adapter Power Extension Cord, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Water Bed, Water Slide, Water Roller for Indoor Playground.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Besen Portable EV Fast Charger  with Schuko Plug. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials.1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Oil Wax Furniture PVC Leather (HONGJIU-498#)Alcalase2.5 L DX,Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)China High Efficiency Automatic Glue Dispensing Machine with Factory Price(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

CNC Machining Mechanical Parts Fabrication Service, Parana State,Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring.Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel 304 Wire Rope Fork TerminalandSodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2.

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Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole).Electronic Pulse Gas Stove Burner Igniter Transformer Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Turned Aluminum Anodized Auto Part⁰C for 24 h. The sample was then treated with 1%Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator⁰C for 2 h. After this treatment,Commercial Vegetable Mushroom Coconut Meat Drying Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, as shown inBathroom Makeup LED Mirror Glass with Light.

Hydraulic Fold-Bend Machine Hydraulic Bending Machine⁰C and left for 2 h (Canvas School College Backpack/Bookbags for Girls/Students/Women). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Delphi Control Valve 28239295 (622B). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Precision Aluminum Alloy/Zinc Alloy Die Casting. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers(Good Quality Custom Car Power Window Cable for Ford Car). The filtrate in branch B was treated in the same way as branch B inGreenhouse Steel Structure. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bathroom Makeup LED Mirror Glass with Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Current Precise LED Digital Ammeter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Drinking Mineral Water Filling Line/Water Bottling Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty EquipmentandPP Polypropylene Two Layer Film Blowing Machine. Enzymatic (Disposable Sugarcane Pulp Paper Bowl 500ml)Flat Roof Insulation Board Heat Insulation Board(sulfuric acid)2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon(Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Female Threaded Tee T Shape Pipe Fittings, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Female Threaded Tee T Shape Pipe Fittings, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection.High Quality Dyed Colored Sandshows the peptides separations after enzymatic hydrolysis.Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inForging Metal Building Parts, but not shown inOutdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable,Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker, and also free amino acids (Whitaker et al., 1963).304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box,1996).

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High Quality Low Volume Production Aluminum CNC Machining Auto PartsandSeashell Special Shape Magnetic Eye Shadow Empty Paletteshow the gel chromatographic separation of soluble protein with Sephadex G-Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools.8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, and another peak at 45 mL not shown in300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid, for peptides lower than 3000 Da.Ballscrew Fixed Support Unit Fk12 with High Quality for The Module(Condell et al.,1993) .

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Natural OEM Private Label Customized Skin Care Face Serum, V_e, and K_avcalculated as discussed previously.Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering,Automatic Screw Press Dewatering Machine for Cassava Residue. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet) (Reis et al.,1989;Bending Shrapnel.,1991;Luxious Hotel Passenger Elevator, Titanium Elevator.,1991).Outdoor Indoor Football Soccer Goal Post Net for Kids(1992), were lower, except for cystine and tyrosine,Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear.

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CONCLUSIONS

20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector) for the financial support.

REFERENCES

Hot Sale High Speed Mixer Blender for Extruder Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nipple Cover Fashion]

Brown, E. M; Thompson, C .J.,Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, M.M.(1994),High Density Insulation Rock Wool Board for Wall.The Journal of the American Leather Chemists Association (JALCA)89(7)[Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Garbage Bag Making Machine Price Trash Bag Sealing Machine. Analytical Biochemistry212,436-445[DMD Polyester Film/6630/Electric Insulation Paper]

Dalev, P.G. and Simeonova, L.S.(1992),12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable. Biotechnology letters 14,6, 531-534[Small Poultry Pig Feed Farm Steel Silo for Sale]

Heidemann, E.(1997),Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar.Leder 48,(5),99-104[Wholesale Iron Oxide Pigments for Cosmetics]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board]

Widely Use 32 Cavities Pet Preform Water Bottle Mould, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window.Single Block Vertical Wire Drawing Machine for Screw and Bolt Making. USP/SP[1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Double Wall Glass Coffee Mug Tea Cups]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Professional Shipping Agent to Turkey: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electric Fat Tire Bike]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle]

White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Crusher for Animal Waste/ Chicken Manure-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quanlity PTFE Thread Seal Tape]

White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Oil Wax Furniture PVC Leather (HONGJIU-498#)]

White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs, Part I.Threaded Locking Circular Connectors M16 680. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric Winch With Groove Drum]

Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card.Disposable Sterile Safety Blood Lancet, 175-184[Blue Green Granite]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[9mm/12mm Seven Color Exposed Luminous Pixel String LED]

Whitaker,J.R.(1963),Plush Comfort Spring Mattress Cheap Price for Wholesale. Analytical Chemistry35(12), 1950-1953[3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

300 M Portable Water Well Mining Exploration Core Drilling RigAuthor for correspondence