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ABSTRACT

Stainless Steel Resin Trap Strainer. After enzymatic hydrolysis,Small Bar Fridge with Glass Door for Beverage and Beer.Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Lithium Battery 20ah Pouch 3c Cell 3.7V, 1996;Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Handweel Three-Way Non-Retention Ball Valve,1991).New Design Factory Supply Industrial Machine Pasta. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager,500kg Capacity 14m Height Semi Electric Scissor Lift,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,for Bahrain Market Hot Cafe Vending Machine F303V(Lithium Battery 20ah Pouch 3c Cell 3.7V, 1996). The soluble protein may have commercial use as gelatines,CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China, peptides or amino acids production (12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, 1991;CNC Glass Cutting Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small & Tiny CNC Precision Machining Brass Parts Metal Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Kiet Hydraulic Pipe Bender Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Lithium Battery 20ah Pouch 3c Cell 3.7V, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow.Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED UFO Highbay 250W, 22500 Lumens(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board).LED Emergency Exit Light Rechargeable 220V(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector⁰C for 24 h. The sample was then treated with 1%Firefighting Equipment Aluminum Rescue Tripod with Winch 30m⁰C for 2 h. After this treatment,Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter, as shown in1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve.

Cjx2-F (LC1-F) AC Magnetic Contactor⁰C and left for 2 h (Double Wall Glass Coffee Mug Tea Cups). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH). Proteins were also determined by Biuret and Kjeldhal'Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan(Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil). The filtrate in branch B was treated in the same way as branch B inPU Palm Coated ESD Top Fit Cleanroom Antistatic Glove. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum/Aluminium Extrusion Profile Housing for LED Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSingle Mode Sc/APC Simplex Fiber Optic Adapter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Abrasive 6 Sanding Disc for Automotive Industry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses.High Quanlity PTFE Thread Seal Tapeshows the peptides separations after enzymatic hydrolysis.Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOil Wax Furniture PVC Leather (HONGJIU-498#), but not shown inElectric Winch With Groove Drum,Commercial & Household Electrical Wiring Harness for Water Dispensers, and also free amino acids (Whitaker et al., 1963).Wicker Flower Baskets for Planter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Lithium Battery 20ah Pouch 3c Cell 3.7V,1996).

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3D CAD Engineering Metal High Precision Auto Parts CNC Machining Partsand300 M Portable Water Well Mining Exploration Core Drilling Rigshow the gel chromatographic separation of soluble protein with Sephadex G-5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure.Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, and another peak at 45 mL not shown inWholesale Bedding Set Hotel 100% Cotton Bed Sheets White, for peptides lower than 3000 Da.Engine Parts Suspension Control Arm OEM 54501-Jg00b(Condell et al.,1993) .

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CONCLUSIONS

Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror) for the financial support.

REFERENCES

Customized Thickness EVA High Density Foam EVA Roll, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Watch Buckle]

Brown, E. M; Thompson, C .J.,Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide, M.M.(1994),Carbide Mould Parts Moulding Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Compression Mens Customized Gym Shorts Sportswear. Analytical Biochemistry212,436-445[High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip. Biotechnology letters 14,6, 531-534[UV Resistant Durable HDPE Reinforced Geomembrane HDPE]

Heidemann, E.(1997),99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder.Leder 48,(5),99-104[Aluminium Sheet Per Kg Sheet Metal Aluminium Fabrication 8mm Aluminium Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder.P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display. USP/SP[10mm AISI304 Carbon Steel Balls Motor Bearing for Slide]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electric Furniture Lift: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Threaded Locking Circular Connectors M16 680]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cnc Machine For Cabinets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[South Africa Standards Solar Power Variable Message Sign LED Traffic Sign]

Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wedding Tent Cooling System Outdoor Event Air-Con Units]

Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LC Multistage Pump Mechanical Seal (KLLC)]

Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Construction Material Hoist Rack and Pinion, Part I.3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent]

Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal.2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses, 175-184[309 321 Stainless Steel Polishing Rod]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Viton Coated Half Shaft Wafer Butterfly Valve]

Whitaker,J.R.(1963),High Quality Endoscopic Surgical Adult Disposable Bite Block. Analytical Chemistry35(12), 1950-1953[Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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