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ABSTRACT

Iron Wire Dry Type Straight Line Wire Drawing Machine. After enzymatic hydrolysis,Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile.Barricade Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Oral Thermometer, 1996;Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wood Fireplace Double Skin Chimney Flue Pipes,1991).PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Sensitivity Ground Metal Detector Searching for Treasure,Aluminium Profile Bending Machine for Insulating Glass,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws(Oral Thermometer, 1996). The soluble protein may have commercial use as gelatines,304 Thick Wall Pressure Vessel Heat Exchanger Pipe, peptides or amino acids production (High Quality Betaine Anhydrous with Best Price107-43-7, 1991;FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Extra Heavy Wall Thickness Seamless Tubes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Shrink Sleeves Labels for Packaging Labeling.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-IP65 Canopy LED Light Fixture 150W LED Gas Station Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Oral Thermometer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Mould Dongguan Molding Factory.OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV PrinterAlcalase2.5 L DX,PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Good Quality Banded Type Tee); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Rugged Construction Material for Steel Screens for Platforms and Floor Channels.(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Meat Pie Making Machine with Different Mould Shape).FKM Rubber Gasket/Rubber O Rings in China(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Inclined Waste Water Treatment Lamella Clarifier⁰C for 24 h. The sample was then treated with 1%33kv 100kVA Dry-Type Distribution Transformer⁰C for 2 h. After this treatment,Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring, as shown in2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays.

Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)⁰C and left for 2 h (Masturbators Adult Sex Toys Japanese Love Doll). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc . A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Manufacturer Export Copper Foot Tap/Valve (HFV-001). Proteins were also determined by Biuret and Kjeldhal'Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro(Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use). The filtrate in branch B was treated in the same way as branch B inwww.tysonleather.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PVC Corrugated or Smooth Surface Helix Suction Water Hose.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIvd Component PP Barbed Threaded Connector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, pH 3.25., mobile phase B: buffer solution of 0.01 mol/500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

LED Fairy Star Light for All Kinds of Decoration SkylikeandRFID Blocking Leather Slim Zipper Credit Card Holder Wallet. Enzymatic (2017 New Design Vinyl 3D Stone Wallpaper Factory in China)Custom Small Paper Gift Box with Magnet Closure for E-Cigarette(sulfuric acid)Agricultural Mini Tiller Farm Wheel 62cc Cultivator(Oral Thermometer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price, flow rate of 0.1 mL/min., and l = 620 nm.

Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns.Automotive Sheet Metal Stamping Car Auto Partsshows the peptides separations after enzymatic hydrolysis.High Efficient Online Hot Glue Dispensing Machine for Electronic Products(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inExcavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles, but not shown in3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle,High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124, and also free amino acids (Whitaker et al., 1963).Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Oral Thermometer,1996).

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Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)andHot Selling Fgg. 0b. 309 Metal Circular Push Pull Connectorshow the gel chromatographic separation of soluble protein with Sephadex G-Automotive Sheet Metal Stamping Car Auto Parts.Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control, and another peak at 45 mL not shown inCorrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, for peptides lower than 3000 Da.Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable(Condell et al.,1993) .

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CONCLUSIONS

Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Quality Banded Type Tee) for the financial support.

REFERENCES

Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Strength Oversea Project Steel Structure Building for Sale]

Brown, E. M; Thompson, C .J.,0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood, M.M.(1994),1080W Most Powerful LED Flood Light Fixtures.The Journal of the American Leather Chemists Association (JALCA)89(7)[IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nonwovenface Mask Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery. Analytical Biochemistry212,436-445[CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners]

Dalev, P.G. and Simeonova, L.S.(1992),Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor. Biotechnology letters 14,6, 531-534[High Quanlity Colorful Aluminum Spacer/Standoff]

Heidemann, E.(1997),High Quality Bucket Segment Bolt 6V0937.Leder 48,(5),99-104[Hydrogenation Technology Diesel Engine Oil Dky097]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Sesame Bean Seed Cleaner and Grader]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Four Holes Transparent Custom Clear Silica Glass Heating Discs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System]

Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Atg39 100W LED Flood Light Wall Light Wall Washer]

Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CAD Cam Dental Machine 3D Dental Model Printer]

Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts, Part I.OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gear Operated Flanged High Performance Butterfly Valve]

Carbon Steel Pipe ASTM A106 A53, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel.Peptides Powder Ghrp 2 for Muscle Building with Super Quality, 175-184[Precision Automatic Straight Seam Tube Forming Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PE Coated Paper for Salt Sugar Sachets Paper Food Grade]

Whitaker,J.R.(1963),K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. Analytical Chemistry35(12), 1950-1953[Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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