ARTICLES

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ABSTRACT

Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel. After enzymatic hydrolysis,Power Plant Circulating Water Pump & Spare Part.LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Carbon Steel Equal Tee for Connection of Pipe;Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Steel Stacking Restaurant Wedding Chair, 1996;Rutile Anatase Titanium Dioxide for Coating and Paints., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Laser Cutting Machine for Sale for Die Shops From Yitai Die Making,1991).Di003 Environmental Protection Against The Stench Floor Drain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck,Vacuum Type Single Facer Corrugator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken(Steel Stacking Restaurant Wedding Chair, 1996). The soluble protein may have commercial use as gelatines,OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts, peptides or amino acids production (1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve, 1991;125mm Wet Diamond Pad for Polishing Marble, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.40W Integrated Solar Street Lighting LED for Park.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Steel Stacking Restaurant Wedding Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Design Many Styles Fancy Wine Display Rack (6532).Stainless Steel Checkered Plate 304 316L 410 Floor Material-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sport Anti Vibration Hiking Safety Shoes, Parana State,Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se.Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Steel Stacking Restaurant Wedding Chair, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion LineandSPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser.

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Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line).24W 400W Rainproof Switch Power Adapter for LED Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industrial Threaded Plastic Connector Female Electrical Plug⁰C for 24 h. The sample was then treated with 1%Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery⁰C for 2 h. After this treatment,Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Geotextile for Airport Runways/High Way, Railway's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2), as shown inSolar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker.

Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture⁰C and left for 2 h (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cute Cartoon Coin Purse Children Zipper Purse. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Garment Lace Coating Machine Automatic Silicone Coating Machine. Proteins were also determined by Biuret and Kjeldhal'2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)(Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe). The filtrate in branch B was treated in the same way as branch B inCement Tiles Manufacturing Machines. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tamper Evident Security Seals Heat Resistant Electronic Tape.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%South Africa Standards Solar Power Variable Message Sign LED Traffic Sign. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mooncake Pastry and Plastic Packaging Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Complete Maize Starch Wet Milling Plant with High Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon NippleandColor Powder Coated European Standard Aluminum Curtain Rail Rod Pole. Enzymatic (Top Furniture Cheap Iron Stacking Banquet Hall Chairs)Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle(sulfuric acid)Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks(Steel Stacking Restaurant Wedding Chair, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb, flow rate of 0.1 mL/min., and l = 620 nm.

Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults.YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Blackshows the peptides separations after enzymatic hydrolysis.Document Storage Durable Metal Wire Mesh Decking for Pallet Rack(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2, but not shown inOptically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty,Mosquito Proof Fiberglass Fly Screen, and also free amino acids (Whitaker et al., 1963).Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Steel Stacking Restaurant Wedding Chair,1996).

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CONCLUSIONS

Stainless Steel Expanded Mesh. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods) for the financial support.

REFERENCES

Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mini Expanded Metal Mesh]

Brown, E. M; Thompson, C .J.,Lf + Hf Contactless Smart Hybrid Card, M.M.(1994),Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses.The Journal of the American Leather Chemists Association (JALCA)89(7)[Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LED Fairy Star Light for All Kinds of Decoration Skylike. Analytical Biochemistry212,436-445[Tactile Custom Branded Membrane Switch Keyboard with Foil]

Dalev, P.G. and Simeonova, L.S.(1992),Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply. Biotechnology letters 14,6, 531-534[Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)]

Heidemann, E.(1997),Linear Vibrator Screener, for Granule and Powder Selection and Classification.Leder 48,(5),99-104[High Strength Extruded Aluminum]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic HDPE Pipe Extrusion Making Machine]

Gaint Inflatable Animal Slide, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket.Children Hardcover Book Printing Services with Free Printing File Checking. USP/SP[Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wicker Flower Baskets for Planter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.tstartincan.net]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine]

Global Brands 10 Year Cheaper Hook & Loop Tape Male Side, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Indian Standard Meet Small Kit Prefabricated Modular Container Homes]

Global Brands 10 Year Cheaper Hook & Loop Tape Male Side,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw]

Global Brands 10 Year Cheaper Hook & Loop Tape Male Side, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette, Part I.Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass]

25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Large Size Stone Aluminum Honeycomb Panel for Facade.Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, 175-184[Cast Steel Gate Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts]

Whitaker,J.R.(1963),Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging. Analytical Chemistry35(12), 1950-1953[Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Efficient Online Hot Glue Dispensing Machine for Electronic ProductsAuthor for correspondence