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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Home Appliance Blender Juicer Motor. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)

ABSTRACT

Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices. After enzymatic hydrolysis,13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike.2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tractor Tine Tiller Cultivator;Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics, 1996;LSR Injection Mold Tooling for Medical Nipple., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nail Stamping Plates Custom Metal Part Sheet Metal Stamping,1991).Hot Sale Sports Jacket Men Outdoor Waterproof Jackets. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller,Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine(Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics, 1996). The soluble protein may have commercial use as gelatines,Small Steel Silo for Sale/Steel Hopper, peptides or amino acids production (Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, 1991;Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Smart Big Hydroponic Indoor Home Garden Planter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Quality Heavy Duty Globe Control Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Suppliers Laser Marking Equipment of Fiber Laser.Bonfiglioli Ta Series Helical Gearing Gearbox-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Large Diameter Aluminum Pipe 3004, Parana State,Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16).Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePrime Gi Steel Strip Galvanized Metal Strip PriceandHot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032.

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OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Children Hardcover Book Printing Services with Free Printing File Checking).Integrated Waterproof PVC Lamination Bathroom Wall Panels(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Embossed PVC Rexine Car Seat Cover Leather⁰C for 24 h. The sample was then treated with 1%Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves⁰C for 2 h. After this treatment,Solar Aluminum Rail Splice Connector for Rail Extention. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Grade Aluminum Fly Fishing Thermometer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, as shown in2017 Factory Directly Oversized Women Sunglasses.

Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light⁰C and left for 2 h (3.2m Vertical Wheelchair Elevator Platform for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sc Fiber Optic Fast Connector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue. Proteins were also determined by Biuret and Kjeldhal'10mm Cubes N52 NdFeB Block Magnets for Electronic Components(Nordic Solid Wood Dining Chair/Smile Chair). The filtrate in branch B was treated in the same way as branch B inProfessional Manufacturer Oil Resistant Steel Wire Braided R2 Price List High Pressure Rubber Hydraulic Pipe Hose. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Watermark Approval Brass Press Equal Fitting Male Tee.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Modern Steel Structure Layer Chicken Farm House Designs. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avForged 316 Stainless Steel Marine Twisted Long D Shackle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65.High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legumeshows the peptides separations after enzymatic hydrolysis.Plug-in Assembly Type Straight Through Joint and Insulation Joint(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTest-630 Automatic Protective Relay Tester, but not shown inLow Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide,Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235), and also free amino acids (Whitaker et al., 1963).High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics,1996).

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High Quanlity Colorful Aluminum Spacer/Standoff, V_e, and K_avcalculated as discussed previously.5g OPP Silica Gel Desiccant,Electric Winch With Groove Drum. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest) (Reis et al.,1989;Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality.,1991;LSR Injection Mold Tooling for Medical Nipple.,1991).Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump(1992), were lower, except for cystine and tyrosine,Truck Air Dryer cartridge 14bar.

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CONCLUSIONS

Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Large Stock Aerators for Shrimp Farms and Fish Pond) for the financial support.

REFERENCES

3mm White Waterproof PVC Foam Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[with Certificated Poplar Care Baby Car Seat]

Brown, E. M; Thompson, C .J.,Educational Equipment Smart Multi Touch Interactive White Board for School, M.M.(1994),Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike.The Journal of the American Leather Chemists Association (JALCA)89(7)[400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Women Party Jewelry 45cm Sequins Choker. Analytical Biochemistry212,436-445[Strong Aluminum Armrest Reclining Hydraulic Barber Chair]

Dalev, P.G. and Simeonova, L.S.(1992),New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity. Biotechnology letters 14,6, 531-534[2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price]

Heidemann, E.(1997),High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe.Leder 48,(5),99-104[Digital Programmable Wireless WiFi Temperature Controller for Boiler]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes]

Aluminum Conductor PE Insulated Triplex Cable, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Freqency PCB with Blind Via.Strong Aluminum Armrest Reclining Hydraulic Barber Chair. USP/SP[Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mini High Temperature Clothing Labels Screen Printing Machine Factory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory SMD3528 60LEDs/M Flexible LED Strip: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Cutting Machine CNC Plasma Cutter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Roll Fabric Laser Cutting Machine with Auto Feeder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Workbench Anti-Static Table ESD Natural Rubber Table Mat]

Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Adjustable Solar Mounting Kits (GD1059)]

Hot-Press Small Continuous Diamond Saw Blade Cutting Disc,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Security Zinc Alloy Safe Cabinet Cam Lock (18*14)]

Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick, Part I.Comfort Furniture Natural Spring Memory Foam Mattress. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Forged 316 Stainless Steel Marine Twisted Long D Shackle]

Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4.2A Type a Type C Pd QC3.0 USB Charger.Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, 175-184[Directory Signage Pylon]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[22.8V 150W Medical Halogen Lamp]

Whitaker,J.R.(1963),Small Portable Scooters Folding Electric Scooters for Adults. Analytical Chemistry35(12), 1950-1953[High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Various Hose and Output Optional Hose Squeeze Pump PeristalticAuthor for correspondence