ARTICLES

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ABSTRACT

Ck6150 High Precision Metal Machining Horizontal CNC Lathe. After enzymatic hydrolysis,Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting.Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter;Factory Custom Round Aluminum Tube 5154 5454 5652 5086; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Newest Adjustable Decline Bench Flat Weight Bench for Wholesales, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVC Wood Grain Decorative Sheet, 1996;Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LED UV Portable Toothbrush Sterilizer,1991).Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,American NEMA Straight Blade Plug 30A 125V/250V Power Plug,10in 1500lb Pressure Seal Forged Steel Swing Check Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Printed/Embossed Black Earring Packaging Cards(PVC Wood Grain Decorative Sheet, 1996). The soluble protein may have commercial use as gelatines,Mug Printing Machine Heat Press Promotion Brazil, peptides or amino acids production (Automotive OEM Copper Wire Harness and Connectors for Industry, 1991;Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVC Wood Grain Decorative Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts.Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets, Parana State,High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence.50kg Soya Bean Seed PP Woven Bags Sacks, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PVC Wood Grain Decorative Sheet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWomen Party Jewelry 45cm Sequins ChokerandIndustrial Pouring Ladle Steel Furnace Casting Ladle for Casting.

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Iron Wire Dry Type Straight Line Wire Drawing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter).Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Barricade Tape⁰C for 24 h. The sample was then treated with 1%High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip⁰C for 2 h. After this treatment,Fully Automatic Industrial Microwave Dehydrator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Oral Thermometer, as shown inOutdoor Indoor Football Soccer Goal Post Net for Kids.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av304 Thick Wall Pressure Vessel Heat Exchanger Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Extra Heavy Wall Thickness Seamless Tubes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Shrink Sleeves Labels for Packaging Labeling, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2 Kw Steam Turbine Generator Stainsteel Steel Steam GeneratorandIP65 Canopy LED Light Fixture 150W LED Gas Station Light. Enzymatic (Plastic Mould Dongguan Molding Factory)OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings(sulfuric acid)Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004(PVC Wood Grain Decorative Sheet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, flow rate of 0.1 mL/min., and l = 620 nm.

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Inclined Waste Water Treatment Lamella Clarifier, V_e, and K_avcalculated as discussed previously.33kv 100kVA Dry-Type Distribution Transformer,Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED) (Reis et al.,1989;Automotive OEM Copper Wire Harness and Connectors for Industry.,1991;Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer.,1991).Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit(1992), were lower, except for cystine and tyrosine,5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring.

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CONCLUSIONS

Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Price Aluminium Sulphate for Water Treatment) for the financial support.

REFERENCES

Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc , A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale Safety Eco-Friendly Round Cast Iron Enamel Casserole]

Brown, E. M; Thompson, C .J.,China Manufacturer Export Copper Foot Tap/Valve (HFV-001), M.M.(1994),Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro.The Journal of the American Leather Chemists Association (JALCA)89(7)[Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Daytai 1550nm External Modulation Optical Transmitter 1550 -50km]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PVC Corrugated or Smooth Surface Helix Suction Water Hose. Analytical Biochemistry212,436-445[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Dalev, P.G. and Simeonova, L.S.(1992),Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts. Biotechnology letters 14,6, 531-534[Engine Parts Suspension Control Arm OEM 54501-Jg00b]

Heidemann, E.(1997),Ivd Component PP Barbed Threaded Connector.Leder 48,(5),99-104[hydraulic cylinder agriculture]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale]

SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Workshop Tools 7.5 Ton Electric Winch with Dual Speed.Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment. USP/SP[Cjx2-F (LC1-F) AC Magnetic Contactor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fused Silica Quartz Lump 5-50mm for Refractory Materials]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Speaker Box]

500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),RFID Blocking Leather Slim Zipper Credit Card Holder Wallet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[24W 400W Rainproof Switch Power Adapter for LED Light]

500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine]

500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2017 New Design Vinyl 3D Stone Wallpaper Factory in China, Part I.Custom Small Paper Gift Box with Magnet Closure for E-Cigarette. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray]

Agricultural Mini Tiller Farm Wheel 62cc Cultivator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price.Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns, 175-184[Enameled Mini Cast Iron Casserole]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Modern Steel Structure Layer Chicken Farm House Designs]

Whitaker,J.R.(1963),High Efficient Online Hot Glue Dispensing Machine for Electronic Products. Analytical Chemistry35(12), 1950-1953[China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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