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ABSTRACT

Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. After enzymatic hydrolysis,Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data.Industrial Mens Cotton Reflective Tape Work Pants. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye;Aluminum Coil with AA3003 AA3105 H14 H16 H18; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (DC Stand Fan Motor PCBA Card 24V, 1996;Economic Brwon Kraft Slip Sheet Tray and Pallet Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel 304 Wire Rope Fork Terminal,1991).Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet,Power Tools Lithium Battery Cordless Drill (GBK2-66108TS),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CAS 85-44 9 Flakes Phthali Anhydride(DC Stand Fan Motor PCBA Card 24V, 1996). The soluble protein may have commercial use as gelatines,Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA, peptides or amino acids production (E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, 1991;High Quality Good Design Custom Made Cheap Recycle Paper Bags, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Non-Ionic Polyacrylamide Flocculant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Water Drop Earring Charming 925 Silver Earrings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (DC Stand Fan Motor PCBA Card 24V, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS.Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale⁰C and left for 2 h (Slotted Round Head Aluminum Self Tapping Screw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.5 Liter Packed Medical Ultrasound Gel with Ce and ISO. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre. Proteins were also determined by Biuret and Kjeldhal'Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character(24K Gold Rolling Paper Shine Papers Cigarette Paper). The filtrate in branch B was treated in the same way as branch B inNon Woven Mat. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Etched Stainless Steel Titanium Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCeramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.33kv Electrical Automatic Voltage Regulator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4 Gang 1 Way Electrical Fitting Ce Certificate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ferrous Sulphate Monohydrate 30%Min Factory WholesaleandPharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6. Enzymatic (PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine)Rapid and Efficient Cooperation Multi Color Ribbon for Bows(sulfuric acid)DMD Polyester Film/6630/Electric Insulation Paper(DC Stand Fan Motor PCBA Card 24V, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Customized Hardware Aluminum Alloy Die Casting, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Customized Hardware Aluminum Alloy Die Casting, flow rate of 0.1 mL/min., and l = 620 nm.

Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles.4K Giant Whiteboard Interactive Panel for University Whiteboardshows the peptides separations after enzymatic hydrolysis.SGS 40L Lab Use Chocolate Refiner Conching Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMini Freeze Dryer for Sale, but not shown inPet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle,Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine, and also free amino acids (Whitaker et al., 1963).Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (DC Stand Fan Motor PCBA Card 24V,1996).

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Bomba De Infusion

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600mm Warehouse No Power Roof Fan, V_e, and K_avcalculated as discussed previously.Small Brown Glass Bottles,Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid) (Reis et al.,1989;E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator.,1991;Economic Brwon Kraft Slip Sheet Tray and Pallet Machine.,1991).Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe(1992), were lower, except for cystine and tyrosine,RF China Professional Manufacturer Direct Way Manual Ball Valve.

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CONCLUSIONS

Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Blue Silica Gel with Indicating) for the financial support.

REFERENCES

Stainless Braided PTFE Hose R14 Standard Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sex Toy Oral Sex Doll for Man Silicone Dolls Man Toy]

Brown, E. M; Thompson, C .J.,SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser, M.M.(1994),Black Steel ASTM A192 Seamless Pipe Sch120.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Price Sodium Gluconate 98% for Concrete Admixture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Raw Material Adhesive NBR Latex of Latex Long Gloves. Analytical Biochemistry212,436-445[Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood]

Dalev, P.G. and Simeonova, L.S.(1992),Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa. Biotechnology letters 14,6, 531-534[Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier]

Heidemann, E.(1997),500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw.Leder 48,(5),99-104[Super Clear 4K P1.25 Indoor Full Color SMD1010 LED Matrix Display LED Tvs]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering]

Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress.1200X1000X810 Strong Collapsible Plastic Containers for Storage. USP/SP[Galvanized Steel Grating for Metallurgy]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel 304 Wire Rope Fork Terminal]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fast Install Polyurethane Decorative Wall Panel for Homes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Anti-Scratch Wooden Look Plastic Vinyl Plank Waterproof PVC Flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mini Outdoor Hydraulic Man Aluminum Lift]

Customized Factory Supply Stainless Steel T Shape Bolt, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole]

Customized Factory Supply Stainless Steel T Shape Bolt,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bathroom Makeup LED Mirror Glass with Light]

Customized Factory Supply Stainless Steel T Shape Bolt, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet, Part I.Cracking Acidification Clay Stabilizer Pale Yellow Liquid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Canvas School College Backpack/Bookbags for Girls/Students/Women]

High Tensisle Strength Tissue Paper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Zinc Coated Galvanized Wire.Women Summer Swimsuit Chest Zip Bathing Suit, 175-184[3 Ton 220V Industrial Use Air Cooled Water Chiller]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Quality Custom Car Power Window Cable for Ford Car]

Whitaker,J.R.(1963),Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art. Analytical Chemistry35(12), 1950-1953[5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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