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ABSTRACT

Custom Female Threaded Tee T Shape Pipe Fittings. After enzymatic hydrolysis,Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection.Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, 1996;Ballscrew Fixed Support Unit Fk12 with High Quality for The Module., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Natural OEM Private Label Customized Skin Care Face Serum,1991).Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Screw Press Dewatering Machine for Cassava Residue,Outdoor Indoor Football Soccer Goal Post Net for Kids,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear(8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, 1996). The soluble protein may have commercial use as gelatines,20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory, peptides or amino acids production (Hot Sale High Speed Mixer Blender for Extruder Machine, 1991;Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Density Insulation Rock Wool Board for Wall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Garbage Bag Making Machine Price Trash Bag Sealing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar.Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Single Block Vertical Wire Drawing Machine for Screw and Bolt Making(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Choline Chloride

Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)).Disposable Sterile Safety Blood Lancet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plush Comfort Spring Mattress Cheap Price for Wholesale⁰C for 24 h. The sample was then treated with 1%3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss⁰C for 2 h. After this treatment,Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, as shown inOffice Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16).

Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain⁰C and left for 2 h (New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Waterproof LED Flash Shoe Light Crystal Epoxy Resin. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Shopping Paper Bag for Clothing Package. Proteins were also determined by Biuret and Kjeldhal'Qingdao Us Type Load Binder Lever Chain Tensioner(Free Sample Cheap Price Plastic Black Underwear Hanger for Sale). The filtrate in branch B was treated in the same way as branch B inPorcelain Fused to Metal Crown Pfm Co-Cr From Shenzhen Minghao Dental Lab. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Elbow SS304 SS316L.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor 15kv Pole Mounted Automatic Circuit Recloser(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.14 Heads Double Door Multihead Weigher⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/White Marble, Carrara White, Bianco Carrara Marble for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Crusher for Animal Waste/ Chicken Manure) for the financial support.

REFERENCES

E6000 Handheld Three Phase Power Quality and Energy Analyzer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Mould Factory/Plastic Mould for Sale/Plastic Mould Injection]

Brown, E. M; Thompson, C .J.,Qg Jg Fg Series Air Dryer Equipment Factory, M.M.(1994),Motor Bearing 6014 2RS Deep Groove Ball Bearing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Test-630 Automatic Protective Relay Tester]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line. Analytical Biochemistry212,436-445[Activated Alumina Used for Catalyst Carrier in Chemical Industry]

Dalev, P.G. and Simeonova, L.S.(1992),Pet Short Fiber Non Woven Geotextile for Landfill Filtration. Biotechnology letters 14,6, 531-534[Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hollow Tube]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set]

Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Maleic Anhydride CAS 108-31-6-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels]

Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films]

Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic HDPE Pipe Extrusion Making Machine, Part I.Jaguar Jb-390 Automatic PCB Loader and Unloader. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile]

Scew Weighing Scale Electronic Counting Scale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Factory Organic Ethyl Ascorbic Acid.Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, 175-184[Hanaulux Blue 80 O. T. Light 21.5V 130W Medical Halogen Bulb Lamp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Strong Aluminum Armrest Reclining Hydraulic Barber Chair]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel MembranesAuthor for correspondence