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ABSTRACT

Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector. After enzymatic hydrolysis,Drive Travel Car Inflatable Bed.Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wheel Loader Transmission Gear Box/Customized Casting Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), 1996;Auto Spare Parts Ignition Coil 22448-2y001., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3D Good Quality Laser Engraving Machine for Souvenirs Shop,1991).Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording,Reverse Flow Configuration Axial Fan,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Handcraft Wood Jewelry Storage Box with Lock and Key(SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), 1996). The soluble protein may have commercial use as gelatines,TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, peptides or amino acids production (Single Spindle Micro CNC Lathe Ck0640, 1991;Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pipe Thermostat with Sensor Ce Certificate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Nitinol Disposable Guide Wire.Breathable Surgical Paper Tape/ Micropore Medical Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Lw250*700 Spiral Discharge Decanter Separator for Water Treatment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter).Single Cheap Custom Festival Woven RFID Fabric Wristband for Events(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Children Gift Outdoor Wooden Rocking Horse Toy⁰C for 24 h. The sample was then treated with 1%Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330⁰C for 2 h. After this treatment,TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, as shown in355nm UV Laser Marking Equipment for Crystal Materials.

China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration⁰C and left for 2 h (EAS Anti-Theft Optical Security Tag for Glasses Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor High Neck Kids Boots Casual Rubber Men Work Boots. Proteins were also determined by Biuret and Kjeldhal'Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)(Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware). The filtrate in branch B was treated in the same way as branch B inStainless Steel 304 Sanitary Food Industry Welding Spring Non Return Check Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Grinding Machining Parts High Precision(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Disposable Clear Take Away Plastic Salad Bowl Forming Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale 25kg 50kg White PP Woven Sack for Flour Feed GrainandBlank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control. Enzymatic (Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP)Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge(sulfuric acid)Carbon Stee Semless Pipe (ASTM A106 Gr B)(SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale, flow rate of 0.1 mL/min., and l = 620 nm.

Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent.Women′s Seamless Strapless Brashows the peptides separations after enzymatic hydrolysis.Hot Selling House Floor Cleaning 360 Spin Mop(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCable Length 11 Gun Safety Padlock, but not shown inJ55 Short Round Thread Seamless Casing Pipe,Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, and also free amino acids (Whitaker et al., 1963).Advanced Unlocking Tool for Locksmith. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH),1996).

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Hsc Heavy Duty 5 Ton Chain Pulley Block, V_e, and K_avcalculated as discussed previously.Outdoor Christmas Decoration Highway Street Light,Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric) (Reis et al.,1989;Single Spindle Micro CNC Lathe Ck0640.,1991;Auto Spare Parts Ignition Coil 22448-2y001.,1991).China Cheap Newest 70*77cm XPE Foam 3D Wallpaper(1992), were lower, except for cystine and tyrosine,48V 200ah UPS Battery Backup for Telecom.

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CONCLUSIONS

450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest) for the financial support.

REFERENCES

Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[20mm Fitness Gym Floor Mat Tile]

Brown, E. M; Thompson, C .J.,5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment, M.M.(1994),Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Bulk Price Yk-11 Powder for Bodybuilding]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Crawler Excavator Track Bolt 3p2274]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-8L Mini Fridge Freezer for Home and Car Fridge. Analytical Biochemistry212,436-445[China Manufacturer Mini Cultivator for Small or Garden Farming]

Dalev, P.G. and Simeonova, L.S.(1992),Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r. Biotechnology letters 14,6, 531-534[700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit]

Heidemann, E.(1997),23m 4 R Arms 2017 New Design Concrete Placing Boom.Leder 48,(5),99-104[Best Price of EPS Foam Sheet Extrusion Lines]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan]

Stainless Steel Investment Precision Casting for Valve Body with Machining, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle.China Factory Offer New Core I7 15.6 Laptop. USP/SP[Factory SMD3528 60LEDs/M Flexible LED Strip]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Color Coated Corrugated Galvanized Roofing Steel Plate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pet Supplies Pet Food Bamboo Plate Rack Three Bowls of Stainless Steel Ceramic Bowls Dog Bowls Pet Bowls]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera]

Mining Machinery Parts Diamond Saw Blade Segments for Marble, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine]

Mining Machinery Parts Diamond Saw Blade Segments for Marble,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods]

Mining Machinery Parts Diamond Saw Blade Segments for Marble, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Plastic Grid for Paver Grass for Gardening, Part I.Explosion Proof Rechargeable LED Searchlight. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Design Felt Belt Type Cooling Table/Handling Extrusion System]

Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair.Soft Rubber Hair Remover Pet Brush, 175-184[304 316 Naked Stainless Steel Banding Strap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Shopping Paper Bag for Clothing Package]

Whitaker,J.R.(1963),Forging Metal Building Parts. Analytical Chemistry35(12), 1950-1953[3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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