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ABSTRACT

Stainless Steel Whisky Chilling Stones. After enzymatic hydrolysis,Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove.CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Solar Panel PV System Mc4 Circular Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, 1996;Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting,1991).Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Recycling Centrifugal Dewatering Machine,Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,EAS Anti-Theft Optical Security Tag for Glasses Bottle(1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, 1996). The soluble protein may have commercial use as gelatines,Single Phase Digital Electric Power Meter, peptides or amino acids production (Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly, 1991;2W 100lm LED Solar Lawn Light for Garden or Yard Lighting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.28mm China High Quality Supplier Screw Cannon Cap for Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure.Nonwovenface Mask Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Professional Indoor&Outdoor Trampoline Park(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment).Transmission Ultrasound Gel /20ml, 250ml, 5L(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire⁰C for 24 h. The sample was then treated with 1%Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence⁰C for 2 h. After this treatment,Food Grade Ivory Paper Cupcake Boxes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Slide Switch - DC Power Jack RoHS's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370, as shown inLinear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet.

2018 New Style High Quality Enamel Bowl with Lid⁰C and left for 2 h (High Density Artificial Grass Football Playground Surfaces (STO)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Roots Vacuum, Vacuum Pump, Pulp Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes. Proteins were also determined by Biuret and Kjeldhal'Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads(Manufacturer High Strength Carbon Steel Heavy Hex Nut). The filtrate in branch B was treated in the same way as branch B inBoat Rubber Side Roll. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEuropean Hotel Decorative Natual Shell Square Standing Floor Lamp(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pengkai Domestic Underground Sewage Treatment Plant Aao Process⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PV Serial Bevel Gearbox for Drilling Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light.A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulbshows the peptides separations after enzymatic hydrolysis.Stainless Steel Art-Screen for Lobby(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, but not shown inSafety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory,Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch, and also free amino acids (Whitaker et al., 1963).Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling,1996).

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CONCLUSIONS

Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door) for the financial support.

REFERENCES

Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[18 Microns LLDPE Stretch Film Various Color Manual Wrap Film]

Brown, E. M; Thompson, C .J.,Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage, M.M.(1994),Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[on-Sale Flat Transparent Glass Jar for Sweet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11. Analytical Biochemistry212,436-445[T Slot Aluminum Extrusion Ob4040b Aluminium Profile]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Sesame Bean Seed Cleaner and Grader. Biotechnology letters 14,6, 531-534[Power Plant Circulating Water Pump & Spare Part]

Heidemann, E.(1997),Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag.Leder 48,(5),99-104[High Quality Big Size Cleaning Cotton Flat Mop for Home Use]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[5ton Mini Crawler Crane]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Solid Carbide Single Flute End Mills Oblique Angle Cutting]

PA Flexible Plastic Corrugated Loom Tubing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape]

PA Flexible Plastic Corrugated Loom Tubing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine]

PA Flexible Plastic Corrugated Loom Tubing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power, Part I.Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[12V Car Cigarette Lighter Adapter Power Extension Cord]

3-Way T Type Elbow Quick Connect Pneumatic Fitting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Superior Quality Soya Protein Food Production Machine Line.Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier, 175-184[Battery Operated Toy Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[BY60100C type metal shaping machine/hydraulic shaper machine]

Whitaker,J.R.(1963),Custom Army Style 100% Polyester Fiber Mens Polo Shirt. Analytical Chemistry35(12), 1950-1953[PE100 Plasitc Pipe for Gas Water Chemcial Sewage System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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