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ABSTRACT

Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard. After enzymatic hydrolysis,Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape.Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Masturbators Adult Sex Toys Japanese Love Doll, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale, 1996;Carbon Steel Spherical Plain Radical Bearings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength,1991).12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment,New Powder Spray Booth for Metal Furniture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Small Tin Box for Condoms(Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale, 1996). The soluble protein may have commercial use as gelatines,Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price, peptides or amino acids production (Petrochemical Loading Platform Folding Stairs, 1991;64mm Double Braid UHMWPE Tugboat Mooring Rope, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rubber Soles Vulcanizing Machine/ Press Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe.40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil⁰C and left for 2 h (External Wall Material Panels/Aluminum Composite Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Custom Round Aluminum Tube 5154 5454 5652 5086. Proteins were also determined by Biuret and Kjeldhal'Newest Adjustable Decline Bench Flat Weight Bench for Wholesales(Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric). The filtrate in branch B was treated in the same way as branch B inFurniture Welded Pipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PVC Wood Grain Decorative Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED UV Portable Toothbrush Sterilizer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.American NEMA Straight Blade Plug 30A 125V/250V Power Plug⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/10in 1500lb Pressure Seal Forged Steel Swing Check Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Printed/Embossed Black Earring Packaging Cards, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable.Fashion Design Sleeveless Fitness Wearshows the peptides separations after enzymatic hydrolysis.3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, but not shown in99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder,Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone, and also free amino acids (Whitaker et al., 1963).China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale,1996).

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Hot Sale Loading Truck with Cummins Engine, V_e, and K_avcalculated as discussed previously.Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets,50kg Soya Bean Seed PP Woven Bags Sacks. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw) (Reis et al.,1989;Petrochemical Loading Platform Folding Stairs.,1991;Carbon Steel Spherical Plain Radical Bearings.,1991).Iron Wire Dry Type Straight Line Wire Drawing Machine(1992), were lower, except for cystine and tyrosine,Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile.

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CONCLUSIONS

Barricade Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping) for the financial support.

REFERENCES

High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electric Drill Tool 12V/14.4V/18V Lithium Cordless Drill with Variable Speed Li-ion Battery Charged Drill Electric Drill]

Brown, E. M; Thompson, C .J.,Fully Automatic Industrial Microwave Dehydrator, M.M.(1994),Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L).The Journal of the American Leather Chemists Association (JALCA)89(7)[Lint Free Nail Gel Polish Remover Nonwoven Wipes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial Threaded Plastic Connector Female Electrical Plug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Oral Thermometer. Analytical Biochemistry212,436-445[Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick]

Dalev, P.G. and Simeonova, L.S.(1992),Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS). Biotechnology letters 14,6, 531-534[Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring]

Heidemann, E.(1997),Wood Fireplace Double Skin Chimney Flue Pipes.Leder 48,(5),99-104[20W All in One Solar Street Light Without Any Cable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pneumatic Cut off Ball Valve with Flange Thread Weld End]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminium Profile Bending Machine for Insulating Glass: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Small First Aid Kit Box Red Waterproof Laminate Bags Medical Kits]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plug-in Assembly Type Straight Through Joint and Insulation Joint]

Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),304 Thick Wall Pressure Vessel Heat Exchanger Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow]

Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3-Way T Type Elbow Quick Connect Pneumatic Fitting]

Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Betaine Anhydrous with Best Price107-43-7, Part I.FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape]

Extra Heavy Wall Thickness Seamless Tubes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Shrink Sleeves Labels for Packaging Labeling.IP65 Canopy LED Light Fixture 150W LED Gas Station Light, 175-184[New Arrival Green Series Glass Cream Bottle&Jar for Cosmetic Packaging (PPC-GB-005)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Soft PVC Granule Used for Refrigerator Door Gasket]

Whitaker,J.R.(1963),Plastic Mould Dongguan Molding Factory. Analytical Chemistry35(12), 1950-1953[China Products Suppliers Camlock Coupling Quick Couplings Female]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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