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ABSTRACT

Cracking Acidification Clay Stabilizer Pale Yellow Liquid. After enzymatic hydrolysis,High Tensisle Strength Tissue Paper.High Zinc Coated Galvanized Wire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Women Summer Swimsuit Chest Zip Bathing Suit;Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Female Header Conn Ptich 1.0mm Connector Btb, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tie Down Nylon Back to Back Hook and Loop Straps, 1996;Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor,1991).Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine,High Quality Warm Paste Packaging Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Resin Trap Strainer(Tie Down Nylon Back to Back Hook and Loop Straps, 1996). The soluble protein may have commercial use as gelatines,Small Bar Fridge with Glass Door for Beverage and Beer, peptides or amino acids production (Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player, 1991;Cheap Loader Xt860 Backhoe Loader with Digger, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lithium Battery 20ah Pouch 3c Cell 3.7V. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tie Down Nylon Back to Back Hook and Loop Straps, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk.Stainless Steel Handweel Three-Way Non-Retention Ball Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Design Factory Supply Industrial Machine Pasta(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, Parana State,Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet.CNC Glass Cutting Line, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Tie Down Nylon Back to Back Hook and Loop Straps, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for SaleandIron Wire Dry Type Straight Line Wire Drawing Machine.

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Ring Retaining

Small & Tiny CNC Precision Machining Brass Parts Metal Part(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall).Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Kiet Hydraulic Pipe Bender Machine⁰C for 24 h. The sample was then treated with 1%PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow⁰C for 2 h. After this treatment,Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LED UFO Highbay 250W, 22500 Lumens's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved, as shown in2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon.

Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine⁰C and left for 2 h (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather. Proteins were also determined by Biuret and Kjeldhal'Cleaning Lint Free Microfiber Mop(Single Cheap Custom Festival Woven RFID Fabric Wristband for Events). The filtrate in branch B was treated in the same way as branch B inBuilding Material PVC 3D Wall Panels / 3D Wall Tiles for 3D Wall Deco. The final soluble protein shown were used for peptides separations using gel permeation chromatography,L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED Emergency Exit Light Rechargeable 220V(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Firefighting Equipment Aluminum Rescue Tripod with Winch 30m⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Bulk Wonderful Buckle Sexy Bikini with Belt SwimwearandAutomatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter. Enzymatic (Cjx2-F (LC1-F) AC Magnetic Contactor)30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal(sulfuric acid)Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)(Tie Down Nylon Back to Back Hook and Loop Straps, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan, flow rate of 0.1 mL/min., and l = 620 nm.

Aluminum/Aluminium Extrusion Profile Housing for LED Light.White Melamine Powder for Industrial Usesshows the peptides separations after enzymatic hydrolysis.Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAir Duct Making Machine, but not shown in64mm Double Braid UHMWPE Tugboat Mooring Rope,Single Mode Sc/APC Simplex Fiber Optic Adapter, and also free amino acids (Whitaker et al., 1963).120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tie Down Nylon Back to Back Hook and Loop Straps,1996).

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CONCLUSIONS

Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (for Bahrain Market Hot Cafe Vending Machine F303V) for the financial support.

REFERENCES

Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Fancy Custom Fur Collar Womens Lady Jacket Wholesale Clothing Down Jackets Women 2017 Winter]

Brown, E. M; Thompson, C .J.,Commercial & Household Electrical Wiring Harness for Water Dispensers, M.M.(1994),Wicker Flower Baskets for Planter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure. Analytical Biochemistry212,436-445[OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings]

Dalev, P.G. and Simeonova, L.S.(1992),Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre. Biotechnology letters 14,6, 531-534[Raw Material Adhesive NBR Latex of Latex Long Gloves]

Heidemann, E.(1997),Engine Parts Suspension Control Arm OEM 54501-Jg00b.Leder 48,(5),99-104[General Use Anatase Titanium Dioxide A100 with Fine Particle Size]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Precision Diamond Wire Saw Rope for Cutting Concrete Stone]

Customized Factory Supply Stainless Steel T Shape Bolt, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat.Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker. USP/SP[Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[HDPE Auto-Deflashing Plastic Bottle Blowing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Steel Round Hole Mesh]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Small Steel Silo for Sale/Steel Hopper]

Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[for Bahrain Market Hot Cafe Vending Machine F303V]

Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[TPU TPR Rubber Band Plastic Extruder Machine]

Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle, Part I.Customized Thickness EVA High Density Foam EVA Roll. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Warm Paste Packaging Machine]

Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Carbide Mould Parts Moulding Parts.Compression Mens Customized Gym Shorts Sportswear, 175-184[Earphone]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2000LTR Horizontal Foam Tank Fire Fighting Suppression]

Whitaker,J.R.(1963),Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip. Analytical Chemistry35(12), 1950-1953[Upper Limbs Machine/Spare Parts for Fitness Equipment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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