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ABSTRACT

High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light. After enzymatic hydrolysis,Stainless Steel Art-Screen for Lobby.Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women;Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, 1996;2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical,1991).Samples Free! ! ! Primary/Remelted/Refined Lead Ingots. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo,120PCS/M Flexible LED Strip Tira De LED Light Strip,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring(Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, 1996). The soluble protein may have commercial use as gelatines,Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch, peptides or amino acids production (Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage, 1991;Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Sesame Bean Seed Cleaner and Grader.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pet Strapping Band Production Line.UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings⁰C and left for 2 h (Outdoor Christmas Decoration Highway Street Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Automatic AAC Concrete Lightweight Block Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel CF8/CF8m Street Elbow Pipe Fittings. Proteins were also determined by Biuret and Kjeldhal'Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding(Disposable Skin Stapler (CSPF-35W)). The filtrate in branch B was treated in the same way as branch B inLed High Bay Light Fixtures. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCorrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Carbon Steel Round/Square Pipe Tube Production Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Two - Way Double Row Thrust Tapered Roller Bearings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power) for the financial support.

REFERENCES

Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Morden Design Prefabricated Steel Building Made of China]

Brown, E. M; Thompson, C .J.,Ride on Toys Riding Animals in The Mall, M.M.(1994),Finely Processed Pneumatic Operated Globe Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tongkat Ali Root Extract 200 1]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Color Coated Corrugated Galvanized Roofing Steel Plate. Analytical Biochemistry212,436-445[Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud]

Dalev, P.G. and Simeonova, L.S.(1992),Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting. Biotechnology letters 14,6, 531-534[Barricade Tape]

Heidemann, E.(1997),Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms.Leder 48,(5),99-104[m.precisionpartscn.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification]

Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Precision Diamond Wire Saw Rope for Cutting Concrete Stone.Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty. USP/SP[Stainless Steel Investment Precision Casting for Valve Body with Machining]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4 Heads High Speed Professional Paper Cardboard Laser Cutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Blince High Torque OMR Hydro Motor for Injection Moulding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Small Tin Box for Condoms]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.apolomed.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201]

6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),TV Tower Antenna Supports-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board]

6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5 Liter Packed Medical Ultrasound Gel with Ce and ISO]

6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, Part I.Retractable Multi Core Cable Reel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Water Filter Cartridge PP Sediment Filter Cartridge]

New Xcm 120 Asphalt Mixing Plant for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Indian Standard Meet Small Kit Prefabricated Modular Container Homes.Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, 175-184[Clear Round Reed Diffuse Glass Bottle for Air Freshener]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet]

Whitaker,J.R.(1963),LED Indoor Light Linear Light Aluminium LED Profile. Analytical Chemistry35(12), 1950-1953[Livining Room Rugs Geometric Patterns Carpets 6′*9′]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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