ARTICLES

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ABSTRACT

Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se. After enzymatic hydrolysis,Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel.Power Plant Circulating Water Pump & Spare Part. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, 1996;Steel Stacking Restaurant Wedding Chair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rutile Anatase Titanium Dioxide for Coating and Paints,1991).Laser Cutting Machine for Sale for Die Shops From Yitai Die Making. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Di003 Environmental Protection Against The Stench Floor Drain,Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vacuum Type Single Facer Corrugator(Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, 1996). The soluble protein may have commercial use as gelatines,Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken, peptides or amino acids production (OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts, 1991;1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;125mm Wet Diamond Pad for Polishing Marble., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-40W Integrated Solar Street Lighting LED for Park. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain.New Design Many Styles Fancy Wine Display Rack (6532)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Checkered Plate 304 316L 410 Floor Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)).Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%24W 400W Rainproof Switch Power Adapter for LED Light⁰C for 24 h. The sample was then treated with 1%Industrial Threaded Plastic Connector Female Electrical Plug⁰C for 2 h. After this treatment,Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Geotextile for Airport Runways/High Way, Railway, as shown inRechargeable Lightweight Running LED Headlamp Torch (SYSG-190219).

Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)⁰C and left for 2 h (China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cute Cartoon Coin Purse Children Zipper Purse. Proteins were also determined by Biuret and Kjeldhal'Garment Lace Coating Machine Automatic Silicone Coating Machine(High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light). The filtrate in branch B was treated in the same way as branch B inCrimping Machine High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tamper Evident Security Seals Heat Resistant Electronic Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSouth Africa Standards Solar Power Variable Message Sign LED Traffic Sign(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mooncake Pastry and Plastic Packaging Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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South Africa Standards Solar Power Variable Message Sign LED Traffic SignandWedding Tent Cooling System Outdoor Event Air-Con Unitsshow the gel chromatographic separation of soluble protein with Sephadex G-Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100).Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board, and another peak at 45 mL not shown inLC Multistage Pump Mechanical Seal (KLLC), for peptides lower than 3000 Da.19m Mobile LED Display Billboard for Advertising Screens(Condell et al.,1993) .

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Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042), V_e, and K_avcalculated as discussed previously.Replica Car Wheels with 20inch for BMW Car,SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Full Viton Coated Half Shaft Wafer Butterfly Valve) (Reis et al.,1989;OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts.,1991;Steel Stacking Restaurant Wedding Chair.,1991).Cooling Tower Replaced Fill Media(1992), were lower, except for cystine and tyrosine,A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow.

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CONCLUSIONS

Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection) for the financial support.

REFERENCES

Stainless Steel Expanded Mesh, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Marking Machine For Steel]

Brown, E. M; Thompson, C .J.,Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2, M.M.(1994),Lf + Hf Contactless Smart Hybrid Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses. Analytical Biochemistry212,436-445[Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway]

Dalev, P.G. and Simeonova, L.S.(1992),LED Fairy Star Light for All Kinds of Decoration Skylike. Biotechnology letters 14,6, 531-534[110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator]

Heidemann, E.(1997),Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply.Leder 48,(5),99-104[Plain Chipboard]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Design Many Styles Fancy Wine Display Rack (6532)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[European Hotel Decorative Natual Shell Square Standing Floor Lamp]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Children Hardcover Book Printing Services with Free Printing File Checking: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4 Gang 1 Way Electrical Fitting Ce Certificate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vertical Automatic Price Milk Packing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale High Speed Mixer Blender for Extruder Machine]

One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Global Brands 10 Year Cheaper Hook & Loop Tape Male Side-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old]

One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material]

One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, Part I.Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2017 New Design Vinyl 3D Stone Wallpaper Factory in China]

Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe.Large Size Stone Aluminum Honeycomb Panel for Facade, 175-184[Flex Led Screen]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity]

Whitaker,J.R.(1963),Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System. Analytical Chemistry35(12), 1950-1953[Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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