ARTICLES

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ABSTRACT

LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering. After enzymatic hydrolysis,PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable.3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps, 1996;Best Price Aluminium Sulphate for Water Treatment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Loading Truck with Cummins Engine,1991).Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,50kg Soya Bean Seed PP Woven Bags Sacks,Iron Wire Dry Type Straight Line Wire Drawing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile(4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps, 1996). The soluble protein may have commercial use as gelatines,Barricade Tape, peptides or amino acids production (High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip, 1991;Fully Automatic Industrial Microwave Dehydrator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Oral Thermometer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wood Fireplace Double Skin Chimney Flue Pipes.PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Sensitivity Ground Metal Detector Searching for Treasure(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Shrink Sleeves Labels for Packaging Labeling(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (28mm China High Quality Supplier Screw Cannon Cap for Bottle).IP65 Canopy LED Light Fixture 150W LED Gas Station Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Mould Dongguan Molding Factory⁰C for 24 h. The sample was then treated with 1%OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings⁰C for 2 h. After this treatment,Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, as shown inNew Design Factory Supply Industrial Machine Pasta.

Good Quality Banded Type Tee⁰C and left for 2 h (Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector. Proteins were also determined by Biuret and Kjeldhal'Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles(Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding). The filtrate in branch B was treated in the same way as branch B inFrp Structure Angle Profiles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Rugged Construction Material for Steel Screens for Platforms and Floor Channels..

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%FKM Rubber Gasket/Rubber O Rings in China. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInclined Waste Water Treatment Lamella Clarifier(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit, pH 3.25., mobile phase B: buffer solution of 0.01 mol/5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

IC LED Wire Loop Height Measuring MicroscopeandSuoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). Enzymatic (Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc )China Manufacturer Export Copper Foot Tap/Valve (HFV-001)(sulfuric acid)Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro(4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PVC Corrugated or Smooth Surface Helix Suction Water Hose, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PVC Corrugated or Smooth Surface Helix Suction Water Hose, flow rate of 0.1 mL/min., and l = 620 nm.

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2017 New Design Vinyl 3D Stone Wallpaper Factory in China, V_e, and K_avcalculated as discussed previously.Custom Small Paper Gift Box with Magnet Closure for E-Cigarette,Agricultural Mini Tiller Farm Wheel 62cc Cultivator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Betaine Anhydrous with Best Price107-43-7) (Reis et al.,1989;High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip.,1991;Best Price Aluminium Sulphate for Water Treatment.,1991).0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price(1992), were lower, except for cystine and tyrosine,Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns.

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CONCLUSIONS

High Efficient Online Hot Glue Dispensing Machine for Electronic Products. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (304 Thick Wall Pressure Vessel Heat Exchanger Pipe) for the financial support.

REFERENCES

High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cheap Glass Flower Vases]

Brown, E. M; Thompson, C .J.,Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship, M.M.(1994),Automotive Sheet Metal Stamping Car Auto Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control. Analytical Biochemistry212,436-445[LED UV Portable Toothbrush Sterilizer]

Dalev, P.G. and Simeonova, L.S.(1992),Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable. Biotechnology letters 14,6, 531-534[Gloss Art Paper Made Packing Package]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminium Profile Bending Machine for Insulating Glass]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Universal Designer Brand Fashion Polarized Men Women Sunglasses]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box]

Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel]

Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation]

Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng, Part I.0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws]

1080W Most Powerful LED Flood Light Fixtures, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery.Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor, 175-184[3000 Series Aluminium Plate]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Women Party Jewelry 45cm Sequins Choker]

Whitaker,J.R.(1963),High Quality Bucket Segment Bolt 6V0937. Analytical Chemistry35(12), 1950-1953[Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Foteck SSR 40da Solid State Power SSR RelayAuthor for correspondence