ARTICLES

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ABSTRACT

Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter. After enzymatic hydrolysis,40W Integrated Solar Street Lighting LED for Park.Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold, 1996;Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods,1991).Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sport Anti Vibration Hiking Safety Shoes,Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine(Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold, 1996). The soluble protein may have commercial use as gelatines,24W 400W Rainproof Switch Power Adapter for LED Light, peptides or amino acids production (Industrial Threaded Plastic Connector Female Electrical Plug, 1991;Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Geotextile for Airport Runways/High Way, Railway.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture.Cute Cartoon Coin Purse Children Zipper Purse-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Garment Lace Coating Machine Automatic Silicone Coating Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Mooncake Pastry and Plastic Packaging Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)).Complete Maize Starch Wet Milling Plant with High Quality(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole⁰C for 24 h. The sample was then treated with 1%Top Furniture Cheap Iron Stacking Banquet Hall Chairs⁰C for 2 h. After this treatment,Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb, as shown inNail Stamping Plates Custom Metal Part Sheet Metal Stamping.

Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults⁰C and left for 2 h (Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Document Storage Durable Metal Wire Mesh Decking for Pallet Rack. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mosquito Proof Fiberglass Fly Screen. Proteins were also determined by Biuret and Kjeldhal'Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)(Seasoned Cast Iron South Africa Three Legged Potjie Pot). The filtrate in branch B was treated in the same way as branch B inManual Punching Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%19m Mobile LED Display Billboard for Advertising Screens. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBaby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cooling Tower Replaced Fill Media, pH 3.25., mobile phase B: buffer solution of 0.01 mol/A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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4 Gang 1 Way Electrical Fitting Ce CertificateandHot Sale High Speed Mixer Blender for Extruder Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Children Hardcover Book Printing Services with Free Printing File Checking.One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, and another peak at 45 mL not shown inInfant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, for peptides lower than 3000 Da.Global Brands 10 Year Cheaper Hook & Loop Tape Male Side(Condell et al.,1993) .

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CONCLUSIONS

Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (South Africa Standards Solar Power Variable Message Sign LED Traffic Sign) for the financial support.

REFERENCES

Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[nbzkls.com]

Brown, E. M; Thompson, C .J.,Making Machine for EVA Soft Hose, M.M.(1994),Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474.The Journal of the American Leather Chemists Association (JALCA)89(7)[Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Maleic Anhydride CAS 108-31-6]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Efficiency Air Cooled Air Source Water Chiller Heat Pump. Analytical Biochemistry212,436-445[Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004]

Dalev, P.G. and Simeonova, L.S.(1992),Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top. Biotechnology letters 14,6, 531-534[One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid]

Heidemann, E.(1997),12oz 16oz Glass Mason Jar with Handle and Straw.Leder 48,(5),99-104[Auto-Recovery Workshop Cleaning Reel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Replica Car Wheels with 20inch for BMW Car]

OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mini Freeze Dryer for Sale.5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber. USP/SP[Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Retro Pipe Fitting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Good Design Custom Made Cheap Recycle Paper Bags]

CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality and Efficient Combustion UHP Graphite Electrode-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning]

CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine]

CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Graphite Ring Good Lubrication, Part I.Flat Head Six-Lobe with Gasket Self Tapping Screws. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized UV Drying Heating Machine for Screen Printer]

DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile.0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting, 175-184[SMT FUJI Nxt W08f Feeder Parts 2adlfa0023 Copy New]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)]

Whitaker,J.R.(1963),Weiying Full Computerized Plain and Invisible Sock knitting Machine. Analytical Chemistry35(12), 1950-1953[High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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