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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Wall Mounting Metal Control Box Control Desk. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Precision Tooling Grinding Machine Part

ABSTRACT

Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks. After enzymatic hydrolysis,Kellogg′s Corn Flakes Cereals Snacks Food Making Machine.300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Various Hose and Output Optional Hose Squeeze Pump Peristaltic;Coill Nail Making Machine/Coil Nail Welding Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2017 Hot Diped Galvanized Welded Gabion Basket, 1996;Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (100%Rayon Red Satin Dyeing Fabric for Women Dress,1991).Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Density Artificial Grass Football Playground Surfaces (STO),High Quality Male Water Quick Release Coupling,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Manufacture Hydraulic Cylinder Tube(2017 Hot Diped Galvanized Welded Gabion Basket, 1996). The soluble protein may have commercial use as gelatines,Chenille Microfiber Mop Head, peptides or amino acids production (HDPE Auto-Deflashing Plastic Bottle Blowing Machine, 1991;Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Industrial Grade Anhydrous Sodium Sulfate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2017 Hot Diped Galvanized Welded Gabion Basket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Low Cost Prefab Steel Poultry Chicken House on Sale.Blue Color Fish Paper/6520/6521 Flexible Composites-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Quality Factory 8ml UV Spider Gel Nail CareAlcalase2.5 L DX,Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Plastic Grip Wood Handle Shovel); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Stainless Steel Rigging Screw, Parana State,Precision Tooling Grinding Machine Part.Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2017 Hot Diped Galvanized Welded Gabion Basket, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCarbon Stee Semless Pipe (ASTM A106 Gr B)andNatural Marble Stone Carving Granite Water Garden Decorative Statue Fountain.

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Security Zinc Alloy Safe Cabinet Cam Lock (18*14)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch).Bubule 17 Inch PP Carry on Kids Travel Luggage for Children(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag⁰C for 24 h. The sample was then treated with 1%Farm Machinery Auto Mechanical Straw Crusher for Poultry⁰C for 2 h. After this treatment,Gloss Art Paper Made Packing Package. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)External Wall Material Panels/Aluminum Composite Sheet, as shown inHub 113-27-41330 for Bulldozer D31p-18 Spare Parts.

Cheap Price Paper Straws Individually Wrapped Drinking Straw⁰C and left for 2 h (Breathable Surgical Paper Tape/ Micropore Medical Tape). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Medical Cable Assembly Wire Harness Manufacturer. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Wood Transport Log/Timber Truck Trailers for Sale(Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ). The filtrate in branch B was treated in the same way as branch B inFashion Simple Design Wholesale Price Wooden with Mable TV Stand for MDF TV Stand (UL-9BE242). The final soluble protein shown were used for peptides separations using gel permeation chromatography,HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Hebei Phosphating Black Heading Machine Drywall Screw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Manufacturer CNC High Precision Machining Metal Parts Customized for Auto⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industry Fluorine Lined Butterfly Valve Lug Type, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine PriceandShenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB. Enzymatic (OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack)Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper(sulfuric acid)Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden(2017 Hot Diped Galvanized Welded Gabion Basket, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%100% PVC Resin PVC Clear Transparent Film for Shower Curtain, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%100% PVC Resin PVC Clear Transparent Film for Shower Curtain, flow rate of 0.1 mL/min., and l = 620 nm.

Hotel Security Improvement Zinc Alloy Door Chain. Swineshows the peptides separations after enzymatic hydrolysis.Anti-Pollution Type 160kn Insulator Porclelain Disc(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlat Head Six-Lobe with Gasket Self Tapping Screws, but not shown inEasy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory,Nickel 200 Floating Ball Valve, and also free amino acids (Whitaker et al., 1963).3-Layer PE Coating Production Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2017 Hot Diped Galvanized Welded Gabion Basket,1996).

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Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set FurnitureandMini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucibleshow the gel chromatographic separation of soluble protein with Sephadex G-Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase.Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, and another peak at 45 mL not shown in2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier, for peptides lower than 3000 Da.Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed(Condell et al.,1993) .

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Wooden Queen/King Size Japan Sofa Bed G1162, V_e, and K_avcalculated as discussed previously.J55 Short Round Thread Seamless Casing Pipe,2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag) (Reis et al.,1989;HDPE Auto-Deflashing Plastic Bottle Blowing Machine.,1991;Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash.,1991).3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube(1992), were lower, except for cystine and tyrosine,Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear.

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CONCLUSIONS

Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Grip Wood Handle Shovel) for the financial support.

REFERENCES

Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Silica Sol]

Brown, E. M; Thompson, C .J.,Dust Hood Fume Extraction Systems for Robotic Laser Cutting, M.M.(1994),IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector.The Journal of the American Leather Chemists Association (JALCA)89(7)[SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Blue Color Fish Paper/6520/6521 Flexible Composites]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nordic Solid Wood Dining Chair/Smile Chair. Analytical Biochemistry212,436-445[Cattle Feed Factory Used Dust Collector and Filter]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet. Biotechnology letters 14,6, 531-534[750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.]

Heidemann, E.(1997),Experienced and Professional Plastic Helmet Mold Manufacturer.Leder 48,(5),99-104[Customized Anodized Turned Aluminum Part Fabrication Service CNC Machining Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw]

Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Private Label OEM Best Cute Waterproof Lip Pluming Gloss.Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display. USP/SP[Garment Lace Coating Machine Automatic Silicone Coating Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Two - Way Double Row Thrust Tapered Roller Bearings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cryogenic Cooling Circulation Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Superior Quality Soya Protein Food Production Machine Line]

High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Waterproof LED Flash Shoe Light Crystal Epoxy Resin]

High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses]

High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tent Windows Use China Made PVC Transparent Clear Mesh Tarp, Part I.13 Oz Flex Banner PVC Frontlit Banner Material. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3mm Indoor Wall Cladding Decoration Aluminum Composite Material]

Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables.Chubby 163cm Fat Big Ass Female Real Sex Doll for Man, 175-184[Factory Wholesale Manual Plastic Baby Bottle Cleaning Brush]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2mm White Coated Paper Cake Boards Jordan]

Whitaker,J.R.(1963),Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V. Analytical Chemistry35(12), 1950-1953[Pipe Thermostat with Sensor Ce Certificate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Plastic Mould Dongguan Molding FactoryAuthor for correspondence