ARTICLES

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ABSTRACT

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Key words;Claus Process Catalyst 4-6 mm;4 Different Ball Silicone Ice Cube Maker; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Washable Durable Hotel Polyester Banquet Table Cloths, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, 1996;Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision,1991).Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aerobic Fermentation Organic Fertilizer Agitator,Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CS1363 a Standard UK 3 Pin Power Plug(16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, 1996). The soluble protein may have commercial use as gelatines,Insulation Anti Earthquake Exterior Wall Foam Board, peptides or amino acids production (Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides, 1991;Easy Soak off OEM 10ml Gel Nail Polish, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Recyclable PU Leather Jewelry Pouch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Activated Alumina Used for Catalyst Carrier in Chemical Industry.High Quality 45W 50W Commercial Recessed COB LED Downlight-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Waterproof BNC Connector for Industry, Parana State,Classic Black Color Commercial Glass Door Multi Deck Refrigerator.Indoor Inkjet Media Material, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Efficiency Air Cooled Air Source Water Chiller Heat Pumpand360 Degree Reciprocating Car Wash Machine.

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Steel Jacket Steam Insulation Pipeline Production Famouse in China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Insulation Anti Earthquake Exterior Wall Foam Board).Solid Steel Hotel Safe (G-42BD classics) for Hotel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c⁰C for 24 h. The sample was then treated with 1%CNC Aluminum Alloy Metal Stamping Processing Services⁰C for 2 h. After this treatment,Puer/off White Melamine Particle Board/MDF Board, White Board. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2000LTR Horizontal Foam Tank Fire Fighting Suppression, as shown inYcom USB Charger and Data Cable with Aluminum for Type C.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Made in China 12 Inch Aluminum Flexible Duct Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Safe Delivery Effective Sarms Mk 677 for Bodybuilding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12# X 14# Electric Galvanized Double Strands Barbed Wire, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer) for the financial support.

REFERENCES

Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gctf Double Induced Roll Dry High Gauss Permanent Drum Magnetic Separator]

Brown, E. M; Thompson, C .J.,Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46, M.M.(1994),China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluno Aluminum Veneer Price Aluminium Curtain Wall Details]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gaint Inflatable Animal Slide. Analytical Biochemistry212,436-445[Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves]

Dalev, P.G. and Simeonova, L.S.(1992),White 4*8FT 18mm PVC Foam Board for Cabinets Use. Biotechnology letters 14,6, 531-534[CNC Grinding Machining Parts High Precision]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality and Efficient Combustion UHP Graphite Electrode]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[GB/T845 Stainless Steel Phillips Pan Head Self Tapping Screw]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt]

Carbon Steel Equal Tee for Connection of Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal]

Carbon Steel Equal Tee for Connection of Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Double Cereal Airtight Food Dispenser]

Carbon Steel Equal Tee for Connection of Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, Part I.Steel Stacking Restaurant Wedding Chair. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line]

Rutile Anatase Titanium Dioxide for Coating and Paints, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Laser Cutting Machine for Sale for Die Shops From Yitai Die Making.Di003 Environmental Protection Against The Stench Floor Drain, 175-184[SAMSUNG]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts]

Whitaker,J.R.(1963),Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck. Analytical Chemistry35(12), 1950-1953[200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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