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ABSTRACT

Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush. After enzymatic hydrolysis,Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory.Masturbators Adult Sex Toys Japanese Love Doll. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, 1996;IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Powder Spray Booth for Metal Furniture,1991).Small Tin Box for Condoms. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price,Petrochemical Loading Platform Folding Stairs,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,64mm Double Braid UHMWPE Tugboat Mooring Rope(12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, 1996). The soluble protein may have commercial use as gelatines,Rubber Soles Vulcanizing Machine/ Press Machine, peptides or amino acids production (Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable, 1991;Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Forged Steel Pressure Sealed Bonnet Globe Valve.Stable Adjustable Monitor Mounts Folding LCD Monitor Stand-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Automatic CNC Centerless Lathe for Peeling Steel Round Bar(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs).High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ck6150 High Precision Metal Machining Horizontal CNC Lathe⁰C for 24 h. The sample was then treated with 1%Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting⁰C for 2 h. After this treatment,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Custom Round Aluminum Tube 5154 5454 5652 5086, as shown inShaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate.

Newest Adjustable Decline Bench Flat Weight Bench for Wholesales⁰C and left for 2 h (High Output Vulcanizing Press Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PVC Wood Grain Decorative Sheet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer. Proteins were also determined by Biuret and Kjeldhal'LED UV Portable Toothbrush Sterilizer(3-Layer PE Coating Production Line). The filtrate in branch B was treated in the same way as branch B inThick Suiting Uniform Fabric. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%American NEMA Straight Blade Plug 30A 125V/250V Power Plug. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10in 1500lb Pressure Seal Forged Steel Swing Check Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mug Printing Machine Heat Press Promotion Brazil⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automotive OEM Copper Wire Harness and Connectors for Industry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Green PVC Coated Front Yard Double Wire Mesh Fence PanelandEn71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692). Enzymatic (LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering)PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable(sulfuric acid)3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts(12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone, flow rate of 0.1 mL/min., and l = 620 nm.

China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker. Win 10shows the peptides separations after enzymatic hydrolysis.Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Super Bass Music TV, but not shown inLow Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box,4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps, and also free amino acids (Whitaker et al., 1963).Best Price Aluminium Sulphate for Water Treatment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike,1996).

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Sy03-05-006 Apple Shape Wallet Silicone Bag Coin PurseandFactory Price High Quality Automatic Egg Tray Egg Carton Making Production Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Sale Loading Truck with Cummins Engine.Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets, and another peak at 45 mL not shown inRustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks, for peptides lower than 3000 Da.50kg Soya Bean Seed PP Woven Bags Sacks(Condell et al.,1993) .

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Iron Wire Dry Type Straight Line Wire Drawing Machine, V_e, and K_avcalculated as discussed previously.Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile,Barricade Tape. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses) (Reis et al.,1989;Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable.,1991;IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment.,1991).High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip(1992), were lower, except for cystine and tyrosine,Fully Automatic Industrial Microwave Dehydrator.

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CONCLUSIONS

Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2018 New Design Red-CNC-006 CNC Machining Aluminum Precision) for the financial support.

REFERENCES

Oral Thermometer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bottled Water Treatment Equipment]

Brown, E. M; Thompson, C .J.,Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), M.M.(1994),Wood Fireplace Double Skin Chimney Flue Pipes.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Speed Auto Corrugated Carton Box Paperboard Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solar DC Water Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material. Analytical Biochemistry212,436-445[Safety Traffic Rubber Roof Support Feet Products]

Dalev, P.G. and Simeonova, L.S.(1992),High Sensitivity Ground Metal Detector Searching for Treasure. Biotechnology letters 14,6, 531-534[300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag]

Heidemann, E.(1997),Aluminium Profile Bending Machine for Insulating Glass.Leder 48,(5),99-104[America Extension Cord]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2194230012 for Mercedes Benz Auto Spare Parts Brake Disc]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Betaine Anhydrous with Best Price107-43-7: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality Banded Type Tee]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Selling America Panel Doors with Good Design]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Natural OEM Private Label Customized Skin Care Face Serum]

FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Extra Heavy Wall Thickness Seamless Tubes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Black Plastic Handle Stainless Steel Kitchen Gadget]

FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply]

FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Shrink Sleeves Labels for Packaging Labeling, Part I.IP65 Canopy LED Light Fixture 150W LED Gas Station Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Shatterproof Gold Rim Wine Glasses]

Plastic Mould Dongguan Molding Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings.Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004, 175-184[Food Steam Jacket Kettle 200L Cooking Kettle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manufacturer Supply Durable Grey Color Non-Slip EVA Mat]

Whitaker,J.R.(1963),Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer. Analytical Chemistry35(12), 1950-1953[16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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