ARTICLES

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ABSTRACT

13.8cm Reed Diffuser Clear Glass Bottle for Aroma. After enzymatic hydrolysis,Metal Cutting Machine CNC Plasma Cutter.Medical Suction Connecting Tube with Yankauer Suction Handle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Surface Mounted Wall Light for Home Decoration Waterproof;Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Self-Propelled Wheel Farm Using 3 Rows Corn Harvester, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Right Angle Worm Gearbox for Food and Beverage and Packaging Machines, 1996;Manufacturer High Strength Carbon Steel Heavy Hex Nut., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine,1991).Stainless Steel Seamless Coil Tube ASTM A249/A269. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT),Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hardware Rigging Forged Red Weldless Pear Shaped Link(Right Angle Worm Gearbox for Food and Beverage and Packaging Machines, 1996). The soluble protein may have commercial use as gelatines,Hot Water Pipe Insulation Steam, peptides or amino acids production (Economy Combo Heat Press for Starter, 1991;Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Grade Beverage Clear PVC Tubing Hose., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Right Angle Worm Gearbox for Food and Beverage and Packaging Machines, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Luxury Plastic Pump Cosmetic Packaging as Airless Bottle.Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Aluminum Sheet Metal Case Parts for Industrial Computer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200).Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder⁰C for 24 h. The sample was then treated with 1%RCA Audio Connector Male Gold-Plated Cable Jack Plug Male⁰C for 2 h. After this treatment,Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Premium quality for Potassium Humate powder, as shown inCleaning Lint Free Microfiber Mop.

Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle⁰C and left for 2 h (China Factory Queen Mattress with Visco Memory Foam and Latex). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Home Design Solid Wood Double Adult Bed Bedroom Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont. Proteins were also determined by Biuret and Kjeldhal'High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns(Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box). The filtrate in branch B was treated in the same way as branch B inWhite Cotton Cloth Loose Flap Polishing Wheel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,304 Precision Professional Dimpled Stainless Steel Plate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Special Shape Spring with Good Quality and Fast Delivery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Better Quality White Coated Fbb Paper Ivory Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Tree Root Grinder Stump Grinder.220V/50Hz Stainless Steel Ozone Generator with 5g/Hshows the peptides separations after enzymatic hydrolysis.Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSolar Panel PV System Mc4 Circular Connector, but not shown inSilver Hammered Round Ball and Natural Stone Necklace,Customization! Bdy380CD Horizontal Directional Drilling Machine, and also free amino acids (Whitaker et al., 1963).V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Right Angle Worm Gearbox for Food and Beverage and Packaging Machines,1996).

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Programmable Salt Spray Test Chamber for Corrosion Testing/Test, V_e, and K_avcalculated as discussed previously.Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%,FKM Bearing Oil Seal /Customized. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors) (Reis et al.,1989;Economy Combo Heat Press for Starter.,1991;Manufacturer High Strength Carbon Steel Heavy Hex Nut.,1991).Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes(1992), were lower, except for cystine and tyrosine,T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile.

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CONCLUSIONS

Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double Coil Control Solenoid Valve of Butterfly Valve) for the financial support.

REFERENCES

Max Twintier Rb441t Using Wire Coils Tw1061t, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[CNC Router with Automatic Contour CCD Camera Rj-1325]

Brown, E. M; Thompson, C .J.,Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm, M.M.(1994),Manufacturer Supply Durable Grey Color Non-Slip EVA Mat.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Feed Storage OEM Grain Storage Silo 1cbm to 200cbm. Analytical Biochemistry212,436-445[Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box. Biotechnology letters 14,6, 531-534[Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine]

Heidemann, E.(1997),Captain Woodwork Sanding Machine/Sanding Table.Leder 48,(5),99-104[Hot Sale Hose Fittings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Shrink Sleeves Labels for Packaging Labeling]

Foteck SSR 40da Solid State Power SSR Relay, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling.Atg39 100W LED Flood Light Wall Light Wall Washer. USP/SP[Stainless Steel Art-Screen for Lobby]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Candy Cooling Tunnel System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden]

Gold Inlaid 24K Gold / Flowers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hydraulic Fold-Bend Machine Hydraulic Bending Machine]

Gold Inlaid 24K Gold / Flowers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Slotted Round Head Aluminum Self Tapping Screw]

Gold Inlaid 24K Gold / Flowers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes, Part I.Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[24K Gold Rolling Paper Shine Papers Cigarette Paper]

Hydraulic Cutter Bread Machine (ZMF-20Y), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board.Yet905 Wireless Keypad Doorbell Kits with Remote Control, 175-184[Jjigsaw Puzzle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ferrous Sulphate Monohydrate 30%Min Factory Wholesale]

Whitaker,J.R.(1963),Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR). Analytical Chemistry35(12), 1950-1953[4K Giant Whiteboard Interactive Panel for University Whiteboard]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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