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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Portable Scooters Folding Electric Scooters for Adults, 1996;Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet,1991).Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Most Popular Split Pressurized Flat Panel Solar Water Heater,Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine(Small Portable Scooters Folding Electric Scooters for Adults, 1996). The soluble protein may have commercial use as gelatines,Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag, peptides or amino acids production (Customized UV Drying Heating Machine for Screen Printer, 1991;99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Portable Scooters Folding Electric Scooters for Adults, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cog Mono Round Display LCD Module.4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pet Strapping Band Production Line).Prime Gi Steel Strip Galvanized Metal Strip Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fireproof Aluminum Curtain Wall Panel⁰C for 24 h. The sample was then treated with 1%Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple⁰C for 2 h. After this treatment,High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Adjustable Grooming Table, as shown in500ml PP Sport Water Bottle with Ball.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%15K High Frequency Online UPS UPS RS232/USB/Snmp Optional. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfesstional Suppliers Yutong Golden Dragon Bus Spare Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/220V/50Hz Stainless Steel Ozone Generator with 5g/H, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada SportsandSelf Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Enzymatic (Luxury Jewelry White Gold Plated Engagement Diamond Ring)Free Sample Cheap Price Plastic Black Underwear Hanger for Sale(sulfuric acid)13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay(Small Portable Scooters Folding Electric Scooters for Adults, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%100% Cotton Face Mask in Guangzhou, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%100% Cotton Face Mask in Guangzhou, flow rate of 0.1 mL/min., and l = 620 nm.

OEM High Pressure Machined Part Die Aluminum Casting.China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Makershows the peptides separations after enzymatic hydrolysis.AISI 430 Ba/2b Embossed Stainless Steel Sheet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRoots Vacuum, but not shown inWire Mesh Welding Size 10mm X 10mm,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, and also free amino acids (Whitaker et al., 1963).Drug Delivery ABS Hospital Medical Treatment Medicine Cart. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Small Portable Scooters Folding Electric Scooters for Adults,1996).

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CONCLUSIONS

Commecial Use Watch Display Cabinet for Watch Store Interior Design. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3.2m Vertical Wheelchair Elevator Platform for Sale) for the financial support.

REFERENCES

Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale Square Shape 40W LED 600X600 Ceiling Panel Light]

Brown, E. M; Thompson, C .J.,Slotted Round Head Aluminum Self Tapping Screw, M.M.(1994),Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sc Fiber Optic Fast Connector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Analytical Biochemistry212,436-445[Carbon Steel Spherical Plain Radical Bearings]

Dalev, P.G. and Simeonova, L.S.(1992),MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker. Biotechnology letters 14,6, 531-534[ Carbon Steel 20g Gr. B Steam Cooling Fin Tube]

Heidemann, E.(1997),Hotbar Reflow Machine for FPC of Camera.Leder 48,(5),99-104[50A Cp Wall-Mounted Solar Charge Controller for Solar System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Factory Price Hg1 Self Adjustable Pipe Tank Rotator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4 Different Ball Silicone Ice Cube Maker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pickled Cucumber Production Line/Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Furniture Welded Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring]

High Quality Washable Durable Hotel Polyester Banquet Table Cloths, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Factory 8ml UV Spider Gel Nail Care]

High Quality Washable Durable Hotel Polyester Banquet Table Cloths,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Home Appliance Blender Juicer Motor]

High Quality Washable Durable Hotel Polyester Banquet Table Cloths, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, Part I.200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic]

Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aerobic Fermentation Organic Fertilizer Agitator.Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, 175-184[Square Threaded Rod]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hotel Security Improvement Zinc Alloy Door Chain]

Whitaker,J.R.(1963),CS1363 a Standard UK 3 Pin Power Plug. Analytical Chemistry35(12), 1950-1953[33kv 100kVA Dry-Type Distribution Transformer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

GRP GratingAuthor for correspondence