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ABSTRACT

Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet. After enzymatic hydrolysis,3-Way T Type Elbow Quick Connect Pneumatic Fitting.China Superior Quality Soya Protein Food Production Machine Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Twisted Annealed Wire for Binding, 1996;Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Fashion Jewellery Lady Eardrop Earrings for Women,1991).High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings,Full Automatic AAC Concrete Lightweight Block Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel CF8/CF8m Street Elbow Pipe Fittings(Twisted Annealed Wire for Binding, 1996). The soluble protein may have commercial use as gelatines,Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, peptides or amino acids production (China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service, 1991;High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Low Volume Production Aluminum CNC Machining Auto Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Twisted Annealed Wire for Binding, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank.Carbon Steel Round/Square Pipe Tube Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Green PVC Coated Front Yard Double Wire Mesh Fence Panel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, Parana State,Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power.300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Twisted Annealed Wire for Binding, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Cameraand750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle.

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Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Sesame Bean Seed Cleaner and Grader).(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw⁰C for 24 h. The sample was then treated with 1%Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes⁰C for 2 h. After this treatment,Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, as shown inOutdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System.

20 Experiences on Plastic Injection Moulding Service⁰C and left for 2 h (Atg39 100W LED Flood Light Wall Light Wall Washer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wind Protection Screen. Wind Dust Netting, Wind Dust Net. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,24K Gold Rolling Paper Shine Papers Cigarette Paper. Proteins were also determined by Biuret and Kjeldhal'Two - Way Double Row Thrust Tapered Roller Bearings(CAD Cam Dental Machine 3D Dental Model Printer). The filtrate in branch B was treated in the same way as branch B inColor Rubber Ring. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ride on Toys Riding Animals in The Mall. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFinely Processed Pneumatic Operated Globe Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Precision Diamond Wire Saw Rope for Cutting Concrete Stone, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Gear Operated Flanged High Performance Butterfly ValveandOptically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty. Enzymatic (Blince High Torque OMR Hydro Motor for Injection Moulding Machine)6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring(sulfuric acid)TV Tower Antenna Supports(Twisted Annealed Wire for Binding, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, flow rate of 0.1 mL/min., and l = 620 nm.

Retractable Multi Core Cable Reel.PE Coated Paper for Salt Sugar Sachets Paper Food Gradeshows the peptides separations after enzymatic hydrolysis.New Xcm 120 Asphalt Mixing Plant for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, but not shown inCustom Die Aluminum Castings,Indian Standard Meet Small Kit Prefabricated Modular Container Homes, and also free amino acids (Whitaker et al., 1963).Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Twisted Annealed Wire for Binding,1996).

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Dry & Wet Concrete Surface Metal Grinding Wing, V_e, and K_avcalculated as discussed previously.Workbench Anti-Static Table ESD Natural Rubber Table Mat,316L 10 Micron Sintered Stainless Steel Filter Disc. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (LED UFO Highbay 250W) (Reis et al.,1989;China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service.,1991;Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid.,1991).Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket(1992), were lower, except for cystine and tyrosine,Abstract Light and Shadow Metal Oil Painting for Deco.

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CONCLUSIONS

Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles) for the financial support.

REFERENCES

Cheapest Container Living House/Office/Kiosk, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fashion Women Purse PU Leather Ladies Purse Cute Zipper Wallet Women Luxury Brand Wallet]

Brown, E. M; Thompson, C .J.,Spin Centrifugal Demister Separator or Column Internal, M.M.(1994),Baby Wet Wipe Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Belt Conveyor in Heavy Duty Industry for Mining]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solar Aluminum Rail Splice Connector for Rail Extention]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump. Analytical Biochemistry212,436-445[Wholesale Fashion Jewellery Lady Eardrop Earrings for Women]

Dalev, P.G. and Simeonova, L.S.(1992),Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric. Biotechnology letters 14,6, 531-534[RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment]

Heidemann, E.(1997),360 Degree Reciprocating Car Wash Machine.Leder 48,(5),99-104[Shanghai Linlang Best Selling Products Black Glass Candle Jar Candle Holder Colored Glass]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glass Disc Suspension Insulators]

PA Flexible Plastic Corrugated Loom Tubing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Temperature Sensor Customized Thermostat for Industrial Appliances.Custom Die Aluminum Castings. USP/SP[RCA Audio Connector Male Gold-Plated Cable Jack Plug Male]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Meat Pie Making Machine with Different Mould Shape: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ABS/PP/PU/PC Injection Molding for Auto Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Non-Ionic Polyacrylamide Flocculant]

Gear Operated Flanged High Performance Butterfly Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine]

Gear Operated Flanged High Performance Butterfly Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles]

Gear Operated Flanged High Performance Butterfly Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, Part I.Wholesale Universal Garden Smart Flower Pot. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair]

Reflective Tape for Vehicle Reflective Markings (tape-02), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),White Crystal Powder Betaine Anhydrous CAS#107-43-7.Creative New Product Coin Holder Keychain, 175-184[A4 button envelope file]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather]

Whitaker,J.R.(1963),Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008. Analytical Chemistry35(12), 1950-1953[Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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