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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-J55 Short Round Thread Seamless Casing Pipe. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine

ABSTRACT

3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube. After enzymatic hydrolysis,Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear.Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation;Dust Hood Fume Extraction Systems for Robotic Laser Cutting; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Nordic Solid Wood Dining Chair/Smile Chair, 1996;Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Experienced and Professional Plastic Helmet Mold Manufacturer,1991).Private Label OEM Best Cute Waterproof Lip Pluming Gloss. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display,Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine(Nordic Solid Wood Dining Chair/Smile Chair, 1996). The soluble protein may have commercial use as gelatines,IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet, peptides or amino acids production (Tent Windows Use China Made PVC Transparent Clear Mesh Tarp, 1991;13 Oz Flex Banner PVC Frontlit Banner Material, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chubby 163cm Fat Big Ass Female Real Sex Doll for Man. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Nordic Solid Wood Dining Chair/Smile Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V.10mm AISI304 Carbon Steel Balls Motor Bearing for Slide-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Different Size of Ring NdFeB Magnet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Drive Travel Car Inflatable Bed, Parana State,2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine.Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Nordic Solid Wood Dining Chair/Smile Chair, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehiclesand10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting.

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China PCBA Supplier Custom Game Controller PCB(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light).TPU TPR Rubber Band Plastic Extruder Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wheel Loader Transmission Gear Box/Customized Casting Parts⁰C for 24 h. The sample was then treated with 1%SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)⁰C for 2 h. After this treatment,Auto Spare Parts Ignition Coil 22448-2y001. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3D Good Quality Laser Engraving Machine for Souvenirs Shop's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories, as shown inOutdoor UV Resistant Decoration Material WPC Exterior Wall Panels.

3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording⁰C and left for 2 h (Lf + Hf Contactless Smart Hybrid Card). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reverse Flow Configuration Axial Fan. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Handcraft Wood Jewelry Storage Box with Lock and Key. Proteins were also determined by Biuret and Kjeldhal'TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit(High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange). The filtrate in branch B was treated in the same way as branch B inLed Digital Alarm Clock. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Single Spindle Micro CNC Lathe Ck0640.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPipe Thermostat with Sensor Ce Certificate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Nitinol Disposable Guide Wire, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Breathable Surgical Paper Tape/ Micropore Medical Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Small Portable Scooters Folding Electric Scooters for AdultsandFashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow. Enzymatic (Crane Hook Qtz250 (7032) with 70m Jib Length)China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System(sulfuric acid)Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest(Nordic Solid Wood Dining Chair/Smile Chair, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, flow rate of 0.1 mL/min., and l = 620 nm.

Online High Sensitivity Checkweigher with Pusher Bar.Hot Selling House Floor Cleaning 360 Spin Mopshows the peptides separations after enzymatic hydrolysis.Piggery Farm Pig/Sow Gestation Equipment From China Factory(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUnderground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket, but not shown inRockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube,Lw250*700 Spiral Discharge Decanter Separator for Water Treatment, and also free amino acids (Whitaker et al., 1963).Single Cheap Custom Festival Woven RFID Fabric Wristband for Events. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Nordic Solid Wood Dining Chair/Smile Chair,1996).

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Custom Men′s Round Neck Raglan Sleeve Sport T-ShirtandTree Root Grinder Stump Grindershow the gel chromatographic separation of soluble protein with Sephadex G-Children Gift Outdoor Wooden Rocking Horse Toy.Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330, and another peak at 45 mL not shown inStainless Steel Whisky Chilling Stones, for peptides lower than 3000 Da.TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass(Condell et al.,1993) .

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CONCLUSIONS

Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories) for the financial support.

REFERENCES

Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[E27 Heat Infrared Lamp Holder Shade Cove]

Brown, E. M; Thompson, C .J.,2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet, M.M.(1994),CNC Grinding Machining Parts High Precision.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Brass Precision CNC Machining Spare Auto Parts with Competitive Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Black Steel ASTM A192 Seamless Pipe Sch120]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Cheap Price Disposable IV Infusion Set with Precise Filter. Analytical Biochemistry212,436-445[Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering]

Dalev, P.G. and Simeonova, L.S.(1992),Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag. Biotechnology letters 14,6, 531-534[New Industrial Wheeled Wooden Shoe Display Fixtures for Shop]

Heidemann, E.(1997),GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve.Leder 48,(5),99-104[Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns]

Wooden Queen/King Size Japan Sofa Bed G1162, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Disposable Clear Take Away Plastic Salad Bowl Forming Machine.Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control. USP/SP[Compact New Design IP65 Toggle Limit Switch]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PE Film Blowing Machine (SJ-1000-1300-1500)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carbon Steel Equal Tee for Connection of Pipe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Color Matching Cabinets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rutile Titanium Dioxide Exterior Emulsion Paint]

Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carbon Stee Semless Pipe (ASTM A106 Gr B)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock]

Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Hole Punching Machine/ CNC Perforation Machine]

Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale, Part I.Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Max Twintier Rb441t Using Wire Coils Tw1061t]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Factory Offer New Core I7 15.6 Laptop]

Whitaker,J.R.(1963),High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts. Analytical Chemistry35(12), 1950-1953[12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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