ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy

ABSTRACT

Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed. After enzymatic hydrolysis,Wooden Queen/King Size Japan Sofa Bed G1162.J55 Short Round Thread Seamless Casing Pipe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), 1996;Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dust Hood Fume Extraction Systems for Robotic Laser Cutting,1991).IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nordic Solid Wood Dining Chair/Smile Chair,Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Experienced and Professional Plastic Helmet Mold Manufacturer(Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), 1996). The soluble protein may have commercial use as gelatines,Private Label OEM Best Cute Waterproof Lip Pluming Gloss, peptides or amino acids production (Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display, 1991;Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tent Windows Use China Made PVC Transparent Clear Mesh Tarp. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰13 Oz Flex Banner PVC Frontlit Banner Material.Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet).Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Drive Travel Car Inflatable Bed⁰C for 24 h. The sample was then treated with 1%Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors⁰C for 2 h. After this treatment,China PCBA Supplier Custom Game Controller PCB. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)TPU TPR Rubber Band Plastic Extruder Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wheel Loader Transmission Gear Box/Customized Casting Parts, as shown inFactory Cheap Price Wholesale Black Nickel Bag O Ring Metal.

SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)⁰C and left for 2 h (Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Auto Spare Parts Ignition Coil 22448-2y001. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3D Good Quality Laser Engraving Machine for Souvenirs Shop. Proteins were also determined by Biuret and Kjeldhal'Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories(High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China). The filtrate in branch B was treated in the same way as branch B inAAC Wall Panels Manual Opening Autoclave Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Reverse Flow Configuration Axial Fan. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHandcraft Wood Jewelry Storage Box with Lock and Key(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Single Spindle Micro CNC Lathe Ck0640⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pipe Thermostat with Sensor Ce Certificate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (10mm AISI304 Carbon Steel Balls Motor Bearing for Slide) for the financial support.

REFERENCES

China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Foldable Flat Packing Pizza Food Box Corrugated Card Paper Box]

Brown, E. M; Thompson, C .J.,XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, M.M.(1994),Outdoor High Neck Kids Boots Casual Rubber Men Work Boots.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061). Analytical Biochemistry212,436-445[Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector. Biotechnology letters 14,6, 531-534[Plastic Grip Wood Handle Shovel]

Heidemann, E.(1997),2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet.Leder 48,(5),99-104[Polished Aluminum Mirror Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto Car Spray Booth From Professional Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ck6150 High Precision Metal Machining Horizontal CNC Lathe]

GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Disposable Clear Take Away Plastic Salad Bowl Forming Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory]

GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control, Part I.Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 New Advertising Inflatable LED Jellyfish Balloon for Sale]

Whitaker,J.R.(1963),Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent. Analytical Chemistry35(12), 1950-1953[Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Manufacturer Garbage IncineratorAuthor for correspondence