ARTICLES

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ABSTRACT

Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy. After enzymatic hydrolysis,Steel Stacking Restaurant Wedding Chair.Rutile Anatase Titanium Dioxide for Coating and Paints. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vacuum Type Single Facer Corrugator, 1996;Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts,1991).1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,125mm Wet Diamond Pad for Polishing Marble,Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,40W Integrated Solar Street Lighting LED for Park(Vacuum Type Single Facer Corrugator, 1996). The soluble protein may have commercial use as gelatines,Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, peptides or amino acids production (New Design Many Styles Fancy Wine Display Rack (6532), 1991;Stainless Steel Checkered Plate 304 316L 410 Floor Material, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vacuum Type Single Facer Corrugator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods.Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sport Anti Vibration Hiking Safety Shoes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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cnmirant

Geotextile for Airport Runways/High Way, Railway(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Medical Packing Self Sealing Sterilization Pouch).Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture⁰C for 24 h. The sample was then treated with 1%Cute Cartoon Coin Purse Children Zipper Purse⁰C for 2 h. After this treatment,Garment Lace Coating Machine Automatic Silicone Coating Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tamper Evident Security Seals Heat Resistant Electronic Tape, as shown inCheap Price Roof Wall PU Sandwich Panel Press.

South Africa Standards Solar Power Variable Message Sign LED Traffic Sign⁰C and left for 2 h (Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels. Proteins were also determined by Biuret and Kjeldhal'Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm(380V 3.0HP Peristaltic Pump Swimming Pool Pump). The filtrate in branch B was treated in the same way as branch B inOEM Shaft CNC Production CNC Engineering CNC Machined Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mooncake Pastry and Plastic Packaging Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Complete Maize Starch Wet Milling Plant with High Quality. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avColor Powder Coated European Standard Aluminum Curtain Rail Rod Pole(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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19m Mobile LED Display Billboard for Advertising Screens.45ton Proof Load Testing Water Bag for Life Boatshows the peptides separations after enzymatic hydrolysis.Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChubby 163cm Fat Big Ass Female Real Sex Doll for Man, but not shown inRubber Tire Plastic Recycling Shredder with Factory Price,Replica Car Wheels with 20inch for BMW Car, and also free amino acids (Whitaker et al., 1963).SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Vacuum Type Single Facer Corrugator,1996).

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Stainless Steel Expanded Mesh, V_e, and K_avcalculated as discussed previously.Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2,Lf + Hf Contactless Smart Hybrid Card. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Smart Big Hydroponic Indoor Home Garden Planter) (Reis et al.,1989;New Design Many Styles Fancy Wine Display Rack (6532).,1991;Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken.,1991).Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses(1992), were lower, except for cystine and tyrosine,LED Fairy Star Light for All Kinds of Decoration Skylike.

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CONCLUSIONS

Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (24W 400W Rainproof Switch Power Adapter for LED Light) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket, M.M.(1994),Children Hardcover Book Printing Services with Free Printing File Checking.The Journal of the American Leather Chemists Association (JALCA)89(7)[Blince High Torque OMR Hydro Motor for Injection Moulding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid. Analytical Biochemistry212,436-445[Nice Quality Coloring Printing Children Board Book Printing]

Dalev, P.G. and Simeonova, L.S.(1992),Global Brands 10 Year Cheaper Hook & Loop Tape Male Side. Biotechnology letters 14,6, 531-534[Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling]

Heidemann, E.(1997),New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw.Leder 48,(5),99-104[Clay Tiles Making Machine with Latest Technology]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mini Double Hole ABS Plastic CNC Machined Parts Importer]

LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette.Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt. USP/SP[12oz 16oz Glass Mason Jar with Handle and Straw]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Printed/Embossed Black Earring Packaging Cards]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Men Aumumn Winter Plus Velvet Thickening Sport Hooded Pullover Suit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Underground Carport]

Large Size Stone Aluminum Honeycomb Panel for Facade, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control]

Large Size Stone Aluminum Honeycomb Panel for Facade,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Commecial Use Watch Display Cabinet for Watch Store Interior Design]

Large Size Stone Aluminum Honeycomb Panel for Facade, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, Part I.Making Machine for EVA Soft Hose. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ce Marked Medical Syringe Pump (WP08N)]

Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Efficiency Air Cooled Air Source Water Chiller Heat Pump.Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, 175-184[Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD]

Whitaker,J.R.(1963),12oz 16oz Glass Mason Jar with Handle and Straw. Analytical Chemistry35(12), 1950-1953[China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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