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ABSTRACT

Handcraft Wood Jewelry Storage Box with Lock and Key. After enzymatic hydrolysis,TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit.Single Spindle Micro CNC Lathe Ck0640. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring;Pipe Thermostat with Sensor Ce Certificate; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring, 1996;Nitinol Disposable Guide Wire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Breathable Surgical Paper Tape/ Micropore Medical Tape,1991).Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Crane Hook Qtz250 (7032) with 70m Jib Length,China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest(Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring, 1996). The soluble protein may have commercial use as gelatines,Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, peptides or amino acids production (Online High Sensitivity Checkweigher with Pusher Bar, 1991;Piggery Farm Pig/Sow Gestation Equipment From China Factory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lw250*700 Spiral Discharge Decanter Separator for Water Treatment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Single Cheap Custom Festival Woven RFID Fabric Wristband for Events.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Children Gift Outdoor Wooden Rocking Horse Toy. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330.TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price).CNC Grinding Machining Parts High Precision(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Cheap Price Disposable IV Infusion Set with Precise Filter⁰C for 24 h. The sample was then treated with 1%Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag⁰C for 2 h. After this treatment,GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disposable Clear Take Away Plastic Salad Bowl Forming Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control, as shown inHand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price.

Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP⁰C and left for 2 h (Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Carbon Stee Semless Pipe (ASTM A106 Gr B). Proteins were also determined by Biuret and Kjeldhal'OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale( Educational Equipment Whiteboard). The filtrate in branch B was treated in the same way as branch B inAirline Pet Carrier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Selling House Floor Cleaning 360 Spin Mop. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSrfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Design Sleeveless Fitness Wear, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DC Colorful Battery Operated Small Hand Fan for Outdoor Use, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray. Agrochemicalsshows the peptides separations after enzymatic hydrolysis.Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSmall Brown Glass Bottles, but not shown inPressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins,5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment, and also free amino acids (Whitaker et al., 1963).Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring,1996).

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CONCLUSIONS

China Plastic Grid for Paver Grass for Gardening. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe) for the financial support.

REFERENCES

Explosion Proof Rechargeable LED Searchlight, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Maize Starch Making Machine Corn Flour Processing Line Centrifugal Sieve Machine Starch]

Brown, E. M; Thompson, C .J.,Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1, M.M.(1994),Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[Advanced Unlocking Tool for Locksmith]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[P4.75 Indoor Single Dual Color Advertising LED Display Billboard]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Soft Rubber Hair Remover Pet Brush. Analytical Biochemistry212,436-445[China Cheap Newest 70*77cm XPE Foam 3D Wallpaper]

Dalev, P.G. and Simeonova, L.S.(1992),Forging Metal Building Parts. Biotechnology letters 14,6, 531-534[500kg Capacity 14m Height Semi Electric Scissor Lift]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Geotextile for Airport Runways/High Way]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Sensitivity Ground Metal Detector Searching for Treasure]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Straw Stainless]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[E6000 Handheld Three Phase Power Quality and Energy Analyzer]

Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools]

Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15), Part I.Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision]

Rubber Tire Plastic Recycling Shredder with Factory Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft.Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile, 175-184[Pendant Lamps Led Lobby Deco]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2018 New Design Red-CNC-006 CNC Machining Aluminum Precision]

Whitaker,J.R.(1963),Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data. Analytical Chemistry35(12), 1950-1953[Unique Best Quality Copper Home Alcohol Distiller for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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