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ABSTRACT

Forging Metal Building Parts. After enzymatic hydrolysis,Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound.Safety Traffic Rubber Roof Support Feet Products. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, 1996;China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor,1991).Rubber Tire Plastic Recycling Shredder with Factory Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft,Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data(Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, 1996). The soluble protein may have commercial use as gelatines,Industrial Mens Cotton Reflective Tape Work Pants, peptides or amino acids production (Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye, 1991;Aluminum Coil with AA3003 AA3105 H14 H16 H18, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.DC Stand Fan Motor PCBA Card 24V.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Economic Brwon Kraft Slip Sheet Tray and Pallet Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel 304 Wire Rope Fork Terminal.Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Good Design Custom Made Cheap Recycle Paper Bags, Parana State,Soft Rubber Hair Remover Pet Brush.Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Whisky Chilling StonesandSteel Grating for Industrial Platform.

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Non-Ionic Polyacrylamide Flocculant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll).Fashion Water Drop Earring Charming 925 Silver Earrings(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS⁰C for 24 h. The sample was then treated with 1%Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks⁰C for 2 h. After this treatment,Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card, as shown in Design Engraved Logo Zipper Puller.

Blue Silica Gel with Indicating⁰C and left for 2 h (China Brass Precision CNC Machining Spare Auto Parts with Competitive Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock. Proteins were also determined by Biuret and Kjeldhal'Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)(Black Steel ASTM A192 Seamless Pipe Sch120). The filtrate in branch B was treated in the same way as branch B inSuitable for Adult or Pediatrics! ! Endoscopy Bite Block with Non-Latex Strap. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Precision Green Sand Casting Ball Valve Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBypass Shower Door Screen with Stainless Steel 304 Frame Hardware(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CAD Cam Dental Machine 3D Dental Model Printer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/900kg Pneumatic Motorcycle Lift, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Power Extension Cord AC Cable Plug for Office Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

SGS 40L Lab Use Chocolate Refiner Conching Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA) for the financial support.

REFERENCES

Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Blasting Media]

Brown, E. M; Thompson, C .J.,Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting, M.M.(1994),Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solar Collector Panel for Pool Heater]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation. Analytical Biochemistry212,436-445[Stainless Steel Checkered Plate 304 316L 410 Floor Material]

Dalev, P.G. and Simeonova, L.S.(1992),5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve. Biotechnology letters 14,6, 531-534[Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage]

Heidemann, E.(1997),600mm Warehouse No Power Roof Fan.Leder 48,(5),99-104[White Paper Cardboard Food Bakery Package with Custom Design]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Top Furniture Cheap Iron Stacking Banquet Hall Chairs]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Cable Assembly Wire Harness Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[insharevape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ASTM A335 Seamless Steel Pipe by Grade P91]

RF China Professional Manufacturer Direct Way Manual Ball Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Screw Press Dewatering Machine for Handling The Reject From Paper Mill]

RF China Professional Manufacturer Direct Way Manual Ball Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character]

RF China Professional Manufacturer Direct Way Manual Ball Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Braided PTFE Hose R14 Standard Pipe, Part I.SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders]

Black Steel ASTM A192 Seamless Pipe Sch120, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Raw Material Adhesive NBR Latex of Latex Long Gloves.Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa, 175-184[Soft Silicone Rubber Insulation High Temperature Custom Cable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet]

Whitaker,J.R.(1963),500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw. Analytical Chemistry35(12), 1950-1953[Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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