ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Slotted Round Head Aluminum Self Tapping Screw. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike

ABSTRACT

Classic Black Color Commercial Glass Door Multi Deck Refrigerator. After enzymatic hydrolysis,MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker.Hotbar Reflow Machine for FPC of Camera. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full Viton Coated Half Shaft Wafer Butterfly Valve;Claus Process Catalyst 4-6 mm; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4 Different Ball Silicone Ice Cube Maker, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Washable Durable Hotel Polyester Banquet Table Cloths, 1996;16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe,1991).200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers,Aerobic Fermentation Organic Fertilizer Agitator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment(High Quality Washable Durable Hotel Polyester Banquet Table Cloths, 1996). The soluble protein may have commercial use as gelatines,CS1363 a Standard UK 3 Pin Power Plug, peptides or amino acids production (Insulation Anti Earthquake Exterior Wall Foam Board, 1991;Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Easy Soak off OEM 10ml Gel Nail Polish., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Recyclable PU Leather Jewelry Pouch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Washable Durable Hotel Polyester Banquet Table Cloths, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11).Activated Alumina Used for Catalyst Carrier in Chemical Industry-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality 45W 50W Commercial Recessed COB LED Downlight(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Speed Auto Corrugated Carton Box Paperboard Production Line, Parana State,Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike.Custom Waterproof BNC Connector for Industry, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Washable Durable Hotel Polyester Banquet Table Cloths, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Tensisle Strength Tissue PaperandChinese Color Steel Glazed Tile Purlin Roll Forming Machine.

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Indoor Inkjet Media Material(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength).Steel Jacket Steam Insulation Pipeline Production Famouse in China(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Solid Steel Hotel Safe (G-42BD classics) for Hotel⁰C for 24 h. The sample was then treated with 1%Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c⁰C for 2 h. After this treatment,CNC Aluminum Alloy Metal Stamping Processing Services. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Puer/off White Melamine Particle Board/MDF Board, White Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, as shown inLeisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest.

2000LTR Horizontal Foam Tank Fire Fighting Suppression⁰C and left for 2 h (Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Spped Weaving Machine 6. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc. Proteins were also determined by Biuret and Kjeldhal'Small Poultry Pig Feed Farm Steel Silo for Sale(Ballscrew Fixed Support Unit Fk12 with High Quality for The Module). The filtrate in branch B was treated in the same way as branch B inTwo Output PVC Spiral Reinfroced Suction Hose Pipe Extrusion machine Line with Spiral Haul-off Tractor Caterpillar and Barrel Type Rotating Winder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMade in China 12 Inch Aluminum Flexible Duct Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Safe Delivery Effective Sarms Mk 677 for Bodybuilding, pH 3.25., mobile phase B: buffer solution of 0.01 mol/12# X 14# Electric Galvanized Double Strands Barbed Wire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

HOWO 420HP Rhd Tractor Truck Head. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine) for the financial support.

REFERENCES

Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine]

Brown, E. M; Thompson, C .J.,Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, M.M.(1994),Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46.The Journal of the American Leather Chemists Association (JALCA)89(7)[Men Casual Short Pants Custom Quality Running Sports Wear Shorts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb. Analytical Biochemistry212,436-445[ Injection Molding]

Dalev, P.G. and Simeonova, L.S.(1992),Gaint Inflatable Animal Slide. Biotechnology letters 14,6, 531-534[12/24V 150ah Power Battery Charger]

Heidemann, E.(1997),White 4*8FT 18mm PVC Foam Board for Cabinets Use.Leder 48,(5),99-104[Epi Fluorescence Microscope High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine]

Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se.Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel. USP/SP[Women Summer Swimsuit Chest Zip Bathing Suit]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Power Plant Circulating Water Pump & Spare Part: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Finely Processed Pneumatic Operated Globe Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.alugrating.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Precision Green Sand Casting Ball Valve Parts]

LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carbon Steel Equal Tee for Connection of Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Spped Weaving Machine 6]

LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Car Parking or Access Control 2.4 GHz Active RFID Card]

LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile, Part I.Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg]

Steel Stacking Restaurant Wedding Chair, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rutile Anatase Titanium Dioxide for Coating and Paints.Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, 175-184[Friendly Cat Scratcher]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line]

Whitaker,J.R.(1963),Di003 Environmental Protection Against The Stench Floor Drain. Analytical Chemistry35(12), 1950-1953[SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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