ARTICLES

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^IICNPq/RHAE/biotechnology-High Quality Anti-Corrosion Fiberglass Sheet and Roll. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter

ABSTRACT

Full-Automatic Folding Napkin Paper Machine. After enzymatic hydrolysis,Customized Frame Mount Highway Pegs Enclosure Parts.Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cattle Feed Factory Used Dust Collector and Filter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, 1996;Group 152cm Picnic Folding in Half Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tactile Custom Branded Membrane Switch Keyboard with Foil,1991).304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America,Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets(Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, 1996). The soluble protein may have commercial use as gelatines,Australia Two Pins Power Cord with SAA Certification, peptides or amino acids production (87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger, 1991;Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pickled Cucumber Production Line/Making Machine.Rectangular Lunch Tin Box with Handle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Inflatable Cheering Stick for Promotional Gift(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, Parana State,Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter.Mini Outdoor Hydraulic Man Aluminum Lift, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAlumina Spheres Ceramic Inert Balls as Catalyst Support/CoveringandGalvanized Steel Grating for Metallurgy.

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Cheap Price Roof Wall PU Sandwich Panel Press(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2).Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%industrial aluminum foil roll 8011 8079 1235⁰C for 24 h. The sample was then treated with 1%Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval⁰C for 2 h. After this treatment,High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Afraic Market Mobile Food Vending Cart, as shown inMini Outdoor Hydraulic Man Aluminum Lift.

Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders⁰C and left for 2 h (Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Double Wall Glass Coffee Mug Tea Cups. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning. Proteins were also determined by Biuret and Kjeldhal'30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care(Bathroom Makeup LED Mirror Glass with Light). The filtrate in branch B was treated in the same way as branch B inAstm A563 Nut Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Vibrating Condom Ring and Cock Ring Supplier.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Color Print Glossy Laminated PP Woven Shopping Gift Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wall Mounting Metal Control Box Control Desk, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Canvas School College Backpack/Bookbags for Girls/Students/WomenandPrecision Tooling Grinding Machine Part. Enzymatic (Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks)Kellogg′s Corn Flakes Cereals Snacks Food Making Machine(sulfuric acid)300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid(Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Various Hose and Output Optional Hose Squeeze Pump Peristaltic, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Various Hose and Output Optional Hose Squeeze Pump Peristaltic, flow rate of 0.1 mL/min., and l = 620 nm.

Coill Nail Making Machine/Coil Nail Welding Machine.Good Quality Custom Car Power Window Cable for Ford Carshows the peptides separations after enzymatic hydrolysis.Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment, but not shown inHigh Quality Dyed Colored Sand,2017 Hot Diped Galvanized Welded Gabion Basket, and also free amino acids (Whitaker et al., 1963).Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box,1996).

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Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber CableandAutomotive OEM Copper Wire Harness and Connectors for Industryshow the gel chromatographic separation of soluble protein with Sephadex G-100%Rayon Red Satin Dyeing Fabric for Women Dress.Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, and another peak at 45 mL not shown inHigh Quality Low Volume Production Aluminum CNC Machining Auto Parts, for peptides lower than 3000 Da.High Density Artificial Grass Football Playground Surfaces (STO)(Condell et al.,1993) .

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High Quality Male Water Quick Release Coupling, V_e, and K_avcalculated as discussed previously.Professional Manufacture Hydraulic Cylinder Tube,Chenille Microfiber Mop Head. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid) (Reis et al.,1989;87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger.,1991;Group 152cm Picnic Folding in Half Table.,1991).HDPE Auto-Deflashing Plastic Bottle Blowing Machine(1992), were lower, except for cystine and tyrosine,Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable.

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CONCLUSIONS

High Quality Industrial Grade Anhydrous Sodium Sulfate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2) for the financial support.

REFERENCES

Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[UL62 3c 12AWG Rubber Jacket Power Cable S]

Brown, E. M; Thompson, C .J.,316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, M.M.(1994),Low Cost Prefab Steel Poultry Chicken House on Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Blue Color Fish Paper/6520/6521 Flexible Composites. Analytical Biochemistry212,436-445[Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler]

Dalev, P.G. and Simeonova, L.S.(1992),Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass. Biotechnology letters 14,6, 531-534[Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes]

Heidemann, E.(1997),High Quality Factory 8ml UV Spider Gel Nail Care.Leder 48,(5),99-104[Metal Business Card Holder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DMD Polyester Film/6630/Electric Insulation Paper]

Fully Automatic Digital Pid Temperature Controller, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers.Plastic Grip Wood Handle Shovel. USP/SP[Small Poultry Pig Feed Farm Steel Silo for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[febwebbing.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Double Wall Glass Coffee Mug Tea Cups]

Stainless Steel Rigging Screw, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil]

Stainless Steel Rigging Screw,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle]

Stainless Steel Rigging Screw, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Security Zinc Alloy Safe Cabinet Cam Lock (18*14), Part I.Bubule 17 Inch PP Carry on Kids Travel Luggage for Children. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quanlity PTFE Thread Seal Tape]

300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Farm Machinery Auto Mechanical Straw Crusher for Poultry.Gloss Art Paper Made Packing Package, 175-184[Hand Winch Wire Rope Manual Industrial Winch]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Oil Wax Furniture PVC Leather (HONGJIU-498#)]

Whitaker,J.R.(1963),3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist. Analytical Chemistry35(12), 1950-1953[Electric Winch With Groove Drum]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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