ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Extra Heavy Wall Thickness Seamless Tubes

ABSTRACT

Shrink Sleeves Labels for Packaging Labeling. After enzymatic hydrolysis,IP65 Canopy LED Light Fixture 150W LED Gas Station Light.Plastic Mould Dongguan Molding Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings;Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, 1996;Good Quality Banded Type Tee., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls,1991).Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles,Rugged Construction Material for Steel Screens for Platforms and Floor Channels.,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,FKM Rubber Gasket/Rubber O Rings in China(PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, 1996). The soluble protein may have commercial use as gelatines,Inclined Waste Water Treatment Lamella Clarifier, peptides or amino acids production (33kv 100kVA Dry-Type Distribution Transformer, 1991;Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc .China Manufacturer Export Copper Foot Tap/Valve (HFV-001)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

PVC Corrugated or Smooth Surface Helix Suction Water HoseAlcalase2.5 L DX,Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Ivd Component PP Barbed Threaded Connector); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Workshop Tools 7.5 Ton Electric Winch with Dual Speed(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Density Insulation Rock Wool Board for Wall).RFID Blocking Leather Slim Zipper Credit Card Holder Wallet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2017 New Design Vinyl 3D Stone Wallpaper Factory in China⁰C for 24 h. The sample was then treated with 1%Custom Small Paper Gift Box with Magnet Closure for E-Cigarette⁰C for 2 h. After this treatment,Agricultural Mini Tiller Farm Wheel 62cc Cultivator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns, as shown inTop Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports.

High Efficient Online Hot Glue Dispensing Machine for Electronic Products⁰C and left for 2 h (Hotbar Reflow Machine for FPC of Camera). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship. Proteins were also determined by Biuret and Kjeldhal'Automotive Sheet Metal Stamping Car Auto Parts(Food Grade Beverage Clear PVC Tubing Hose). The filtrate in branch B was treated in the same way as branch B inNew and Modern Design Steel Cupboard Mobile Filing Cabinet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Patient Hoist Lift⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor.Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Lockershows the peptides separations after enzymatic hydrolysis.High Quality Bucket Segment Bolt 6V0937(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, but not shown inHome Design Solid Wood Double Adult Bed Bedroom Furniture,Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture, and also free amino acids (Whitaker et al., 1963).Outdoor Server Rack Network Data Cabinet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles,1996).

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CONCLUSIONS

K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ivd Component PP Barbed Threaded Connector) for the financial support.

REFERENCES

EPDM, PVC Car or Door Rubber Sealing Strip, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Phase Isolator Switch]

Brown, E. M; Thompson, C .J.,Black Color Tube Eyelet Lift Gas Spring, M.M.(1994),Safety Edge Sensor for Agv Automated Guided Vehicle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Hardware Aluminum Alloy Die Casting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets. Analytical Biochemistry212,436-445[Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter]

Dalev, P.G. and Simeonova, L.S.(1992),Best Design Felt Belt Type Cooling Table/Handling Extrusion System. Biotechnology letters 14,6, 531-534[Firefighting Equipment Aluminum Rescue Tripod with Winch 30m]

Heidemann, E.(1997),Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood.Leder 48,(5),99-104[600w Led Floodlight Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Automatic Snack Food Lab Twin Screw Extruder Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Longzhiyu 13 Years Manufacturer Round Silver/ Bronze Sports Medal Custom Metal Medal Custom Shaped Medals]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass]

Seashell Special Shape Magnetic Eye Shadow Empty Palette, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input]

Seashell Special Shape Magnetic Eye Shadow Empty Palette,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers]

Seashell Special Shape Magnetic Eye Shadow Empty Palette, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, Part I.Nice Quality Coloring Printing Children Board Book Printing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Precision Casting Factory Foundry OEM Ductile Iron Sand Casting]

Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine.Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating, 175-184[Round Straw Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)]

Whitaker,J.R.(1963),Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley. Analytical Chemistry35(12), 1950-1953[Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Large Stock Aerators for Shrimp Farms and Fish PondAuthor for correspondence