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ABSTRACT

High Quality Industrial Grade Anhydrous Sodium Sulfate. After enzymatic hydrolysis,Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension.316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Factory 8ml UV Spider Gel Nail Care, 1996;Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Grip Wood Handle Shovel,1991).2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Rigging Screw,Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Security Zinc Alloy Safe Cabinet Cam Lock (18*14)(High Quality Factory 8ml UV Spider Gel Nail Care, 1996). The soluble protein may have commercial use as gelatines,Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, peptides or amino acids production (300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, 1991;Farm Machinery Auto Mechanical Straw Crusher for Poultry, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gloss Art Paper Made Packing Package., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-External Wall Material Panels/Aluminum Composite Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Factory 8ml UV Spider Gel Nail Care, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cheap Price Paper Straws Individually Wrapped Drinking Straw.Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Medical Cable Assembly Wire Harness Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Small Bar Fridge with Glass Door for Beverage and Beer).Industry Fluorine Lined Butterfly Valve Lug Type(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB⁰C for 24 h. The sample was then treated with 1%OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack⁰C for 2 h. After this treatment,Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100% PVC Resin PVC Clear Transparent Film for Shower Curtain, as shown inFashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow.

Hotel Security Improvement Zinc Alloy Door Chain⁰C and left for 2 h (Black Plastic GRP FRP PE Pipe Fitting for Water Supply). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anti-Pollution Type 160kn Insulator Porclelain Disc. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Nickel 200 Floating Ball Valve. Proteins were also determined by Biuret and Kjeldhal'3-Layer PE Coating Production Line(IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet). The filtrate in branch B was treated in the same way as branch B inMinimalism Small Portable Gold Laser Cutting Machine for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBeach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.J55 Short Round Thread Seamless Casing Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Nordic Solid Wood Dining Chair/Smile Chair.Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14Xshows the peptides separations after enzymatic hydrolysis.Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLithium Battery 20ah Pouch 3c Cell 3.7V, but not shown inNew Powder Spray Booth for Metal Furniture,Experienced and Professional Plastic Helmet Mold Manufacturer, and also free amino acids (Whitaker et al., 1963).Private Label OEM Best Cute Waterproof Lip Pluming Gloss. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Factory 8ml UV Spider Gel Nail Care,1996).

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CONCLUSIONS

Chubby 163cm Fat Big Ass Female Real Sex Doll for Man. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer) for the financial support.

REFERENCES

Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Washable Air Filter G4 Eu4 Supplier & Factory]

Brown, E. M; Thompson, C .J.,10mm AISI304 Carbon Steel Balls Motor Bearing for Slide, M.M.(1994),Different Size of Ring NdFeB Magnet.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Hebei Phosphating Black Heading Machine Drywall Screw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Investment Precision Casting for Valve Body with Machining. Analytical Biochemistry212,436-445[DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw]

Dalev, P.G. and Simeonova, L.S.(1992),Unique Best Quality Copper Home Alcohol Distiller for Sale. Biotechnology letters 14,6, 531-534[High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable]

Heidemann, E.(1997),Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories.Leder 48,(5),99-104[High Quality Lace Sleeveless Tops]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier]

Chenille Microfiber Mop Head, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector.Drive Travel Car Inflatable Bed. USP/SP[UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Black Beans Bag Package Sealing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wheel Loader Transmission Gear Box/Customized Casting Parts]

China PCBA Supplier Custom Game Controller PCB, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),TPU TPR Rubber Band Plastic Extruder Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DC Stand Fan Motor PCBA Card 24V]

China PCBA Supplier Custom Game Controller PCB,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical]

China PCBA Supplier Custom Game Controller PCB, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wheel Loader Transmission Gear Box/Customized Casting Parts, Part I.SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Single Mode Sc/APC Simplex Fiber Optic Adapter]

Auto Spare Parts Ignition Coil 22448-2y001, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D Good Quality Laser Engraving Machine for Souvenirs Shop.Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories, 175-184[Kc Kcc UL Ce GS SAA 12V Lead Acid Sprayer Battery Charger 14.6V 1A 2A]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm]

Whitaker,J.R.(1963),3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording. Analytical Chemistry35(12), 1950-1953[Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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