ARTICLES

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ABSTRACT

PVC Wood Grain Decorative Sheet. After enzymatic hydrolysis,Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer.LED UV Portable Toothbrush Sterilizer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding;American NEMA Straight Blade Plug 30A 125V/250V Power Plug; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10in 1500lb Pressure Seal Forged Steel Swing Check Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Printed/Embossed Black Earring Packaging Cards, 1996;Mug Printing Machine Heat Press Promotion Brazil., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automotive OEM Copper Wire Harness and Connectors for Industry,1991).Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692),LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable(Custom Printed/Embossed Black Earring Packaging Cards, 1996). The soluble protein may have commercial use as gelatines,3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts, peptides or amino acids production (Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone, 1991;China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Price Aluminium Sulphate for Water Treatment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Printed/Embossed Black Earring Packaging Cards, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Loading Truck with Cummins Engine.Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:50kg Soya Bean Seed PP Woven Bags Sacks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Oral Thermometer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved).Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wood Fireplace Double Skin Chimney Flue Pipes⁰C for 24 h. The sample was then treated with 1%PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material⁰C for 2 h. After this treatment,High Sensitivity Ground Metal Detector Searching for Treasure. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminium Profile Bending Machine for Insulating Glass's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, as shown inHigh Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence.

304 Thick Wall Pressure Vessel Heat Exchanger Pipe⁰C and left for 2 h (Bubule 17 Inch PP Carry on Kids Travel Luggage for Children). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Betaine Anhydrous with Best Price107-43-7. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards. Proteins were also determined by Biuret and Kjeldhal'Extra Heavy Wall Thickness Seamless Tubes(Tamper Evident Security Seals Heat Resistant Electronic Tape). The filtrate in branch B was treated in the same way as branch B in30w Hanging Led Linear Tube Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Shrink Sleeves Labels for Packaging Labeling.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%IP65 Canopy LED Light Fixture 150W LED Gas Station Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Mould Dongguan Molding Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PVC Wood Grain Decorative SheetandGood Quality Banded Type Tee. Enzymatic (Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls)Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector(sulfuric acid)Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles(Custom Printed/Embossed Black Earring Packaging Cards, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rugged Construction Material for Steel Screens for Platforms and Floor Channels., flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rugged Construction Material for Steel Screens for Platforms and Floor Channels., flow rate of 0.1 mL/min., and l = 620 nm.

FKM Rubber Gasket/Rubber O Rings in China.Boat Deck Drainage Snow Rubber Flooring/Standing Desk Matshows the peptides separations after enzymatic hydrolysis.Inclined Waste Water Treatment Lamella Clarifier(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, but not shown inQueene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike,33kv 100kVA Dry-Type Distribution Transformer, and also free amino acids (Whitaker et al., 1963).Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Printed/Embossed Black Earring Packaging Cards,1996).

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CONCLUSIONS

Ivd Component PP Barbed Threaded Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Barricade Tape) for the financial support.

REFERENCES

Workshop Tools 7.5 Ton Electric Winch with Dual Speed, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter]

Brown, E. M; Thompson, C .J.,Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, M.M.(1994),Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dry & Wet Concrete Surface Metal Grinding Wing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle. Analytical Biochemistry212,436-445[Luxury Plastic Pump Cosmetic Packaging as Airless Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),RFID Blocking Leather Slim Zipper Credit Card Holder Wallet. Biotechnology letters 14,6, 531-534[Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool]

Heidemann, E.(1997),2017 New Design Vinyl 3D Stone Wallpaper Factory in China.Leder 48,(5),99-104[CNC Lathe Machining Gear Shaft with OEM and Customized Services]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SGS 40L Lab Use Chocolate Refiner Conching Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Patten Custom Jacquard Design Fall Boutique Knitted Cotton Kid Vest for Infant]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Carbon Stee Semless Pipe (ASTM A106 Gr B)]

Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Efficient Online Hot Glue Dispensing Machine for Electronic Products-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain]

Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine]

Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124, Part I.Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch]

Automotive Sheet Metal Stamping Car Auto Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control.Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable, 175-184[Car Door Panel Molding Parts Check Fixture]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts]

Whitaker,J.R.(1963),2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1. Analytical Chemistry35(12), 1950-1953[Breathable Surgical Paper Tape/ Micropore Medical Tape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc Author for correspondence