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ABSTRACT

Black Color Tube Eyelet Lift Gas Spring. After enzymatic hydrolysis,Safety Edge Sensor for Agv Automated Guided Vehicle.Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ycom USB Charger and Data Cable with Aluminum for Type C, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine, 1996;8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Seashell Special Shape Magnetic Eye Shadow Empty Palette,1991).on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office,Nice Quality Coloring Printing Children Board Book Printing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808)(Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine, 1996). The soluble protein may have commercial use as gelatines,Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine, peptides or amino acids production (Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating, 1991;Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5km City Surveillance Network Infrared Laser PTZ Camera.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-300 M Portable Water Well Mining Exploration Core Drilling Rig. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Foteck SSR 40da Solid State Power SSR Relay.2015 New Square Folding Ajustable Wall LED Bathroom Mirror-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Right Angle Worm Gearbox for Food and Beverage and Packaging Machines(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw).Manufacturer High Strength Carbon Steel Heavy Hex Nut(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine⁰C for 24 h. The sample was then treated with 1%Stainless Steel Seamless Coil Tube ASTM A249/A269⁰C for 2 h. After this treatment,The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hardware Rigging Forged Red Weldless Pear Shaped Link, as shown inHigh Quality HDMI to VGA Converter Cable with Audio and USB Power Cable.

Hot Water Pipe Insulation Steam⁰C and left for 2 h (Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Economy Combo Heat Press for Starter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler. Proteins were also determined by Biuret and Kjeldhal'Food Grade Beverage Clear PVC Tubing Hose(UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen). The filtrate in branch B was treated in the same way as branch B inFloat Glass Tempered Glass Laminated Glass Insulated Glass Decorative Glass Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLuxury Plastic Pump Cosmetic Packaging as Airless Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/45ton Proof Load Testing Water Bag for Life Boat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper OutwearandDouble Coil Control Solenoid Valve of Butterfly Valve. Enzymatic (High Frequency Online UPS W/3W-20K)Solar Collector Panel for Pool Heater(sulfuric acid)ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers(Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Sheet Metal Case Parts for Industrial Computer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Sheet Metal Case Parts for Industrial Computer, flow rate of 0.1 mL/min., and l = 620 nm.

Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motorshows the peptides separations after enzymatic hydrolysis.High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWheel Loader Transmission Gear Box/Customized Casting Parts, but not shown inDC Stand Fan Motor PCBA Card 24V,RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, and also free amino acids (Whitaker et al., 1963).Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine,1996).

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Single Mode Sc/APC Simplex Fiber Optic AdapterandL2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mmshow the gel chromatographic separation of soluble protein with Sephadex G-A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb.Premium quality for Potassium Humate powder, and another peak at 45 mL not shown inPromotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), for peptides lower than 3000 Da.Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle(Condell et al.,1993) .

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Home Design Solid Wood Double Adult Bed Bedroom Furniture, V_e, and K_avcalculated as discussed previously.Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont,High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Amplifier Tube Glass Vacuum Tube (833C)) (Reis et al.,1989;Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating.,1991;8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input.,1991).304 Precision Professional Dimpled Stainless Steel Plate(1992), were lower, except for cystine and tyrosine,Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence.

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CONCLUSIONS

Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Medical Suction Connecting Tube with Yankauer Suction Handle) for the financial support.

REFERENCES

Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Low Price Aluminum Alloy Printing Screen Frame]

Brown, E. M; Thompson, C .J.,Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport, M.M.(1994),Special Shape Spring with Good Quality and Fast Delivery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Better Quality White Coated Fbb Paper Ivory Board. Analytical Biochemistry212,436-445[Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye]

Dalev, P.G. and Simeonova, L.S.(1992),Excellent Plastic Extruder in Pet PP Packing Strap Machine. Biotechnology letters 14,6, 531-534[Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300]

Heidemann, E.(1997),Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China.Leder 48,(5),99-104[Nmsafety 3 Layer Winter Use PVC Coated Safety Work Glove]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Most Popular Split Pressurized Flat Panel Solar Water Heater]

Peptides Powder Ghrp 2 for Muscle Building with Super Quality, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%.Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. USP/SP[Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 New Advertising Inflatable LED Jellyfish Balloon for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[24 Inch Butterfly Valve Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industry Fluorine Lined Butterfly Valve Lug Type]

Tree Root Grinder Stump Grinder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine]

Tree Root Grinder Stump Grinder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[The Most Demanded Products Fluorescence Microscope Camera]

Tree Root Grinder Stump Grinder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customization! Bdy380CD Horizontal Directional Drilling Machine, Part I.V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine]

Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment.Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification, 175-184[Custom Woven Labels]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping]

Whitaker,J.R.(1963),Programmable Salt Spray Test Chamber for Corrosion Testing/Test. Analytical Chemistry35(12), 1950-1953[Single Block Vertical Wire Drawing Machine for Screw and Bolt Making]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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