ARTICLES

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ABSTRACT

Oral Thermometer. After enzymatic hydrolysis,Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS).Wood Fireplace Double Skin Chimney Flue Pipes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material;High Sensitivity Ground Metal Detector Searching for Treasure; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminium Profile Bending Machine for Insulating Glass, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, 1996;304 Thick Wall Pressure Vessel Heat Exchanger Pipe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Betaine Anhydrous with Best Price107-43-7,1991).FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Extra Heavy Wall Thickness Seamless Tubes,Shrink Sleeves Labels for Packaging Labeling,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,IP65 Canopy LED Light Fixture 150W LED Gas Station Light(Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, 1996). The soluble protein may have commercial use as gelatines,Plastic Mould Dongguan Molding Factory, peptides or amino acids production (OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings, 1991;Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Quality Banded Type Tee. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls.Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap).Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ⁰C for 24 h. The sample was then treated with 1%China Manufacturer Export Copper Foot Tap/Valve (HFV-001)⁰C for 2 h. After this treatment,Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVC Corrugated or Smooth Surface Helix Suction Water Hose's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts, as shown inWholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle.

Ivd Component PP Barbed Threaded Connector⁰C and left for 2 h (Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Workshop Tools 7.5 Ton Electric Winch with Dual Speed. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment. Proteins were also determined by Biuret and Kjeldhal'Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim(Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator). The filtrate in branch B was treated in the same way as branch B inCopper Fencing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%RFID Blocking Leather Slim Zipper Credit Card Holder Wallet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2017 New Design Vinyl 3D Stone Wallpaper Factory in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Agricultural Mini Tiller Farm Wheel 62cc Cultivator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Aluminium Profile Bending Machine for Insulating GlassandSpeedypet Fashionable New Design Oval Pet Products Cat Toilet Boxshow the gel chromatographic separation of soluble protein with Sephadex G-CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23).Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine, and another peak at 45 mL not shown inMulti Purpose Microfiber Towel Car / Home / Gift / Sport Towel, for peptides lower than 3000 Da.Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering(Condell et al.,1993) .

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CONCLUSIONS

High Quality Bucket Segment Bolt 6V0937. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Inclined Waste Water Treatment Lamella Clarifier) for the financial support.

REFERENCES

Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nylon and Polyester Monofilament Mesh (TYC-NMO200)]

Brown, E. M; Thompson, C .J.,Outdoor Server Rack Network Data Cabinet, M.M.(1994),Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera.The Journal of the American Leather Chemists Association (JALCA)89(7)[Foteck SSR 40da Solid State Power SSR Relay]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House. Analytical Biochemistry212,436-445[Outdoor Indoor Football Soccer Goal Post Net for Kids]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable. Biotechnology letters 14,6, 531-534[Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)]

Heidemann, E.(1997),Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts.Leder 48,(5),99-104[Wire Mesh Portable Supermarket Storage Shopping Basket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Black Color Tube Eyelet Lift Gas Spring]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Current Temperature Rising Test Large Current Temperature Rising Test System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[C/U Channel Beam Steel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine]

Peptides Powder Ghrp 2 for Muscle Building with Super Quality, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger]

Peptides Powder Ghrp 2 for Muscle Building with Super Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3mm White Waterproof PVC Foam Board]

Peptides Powder Ghrp 2 for Muscle Building with Super Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:EPDM, PVC Car or Door Rubber Sealing Strip, Part I.Black Color Tube Eyelet Lift Gas Spring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine]

Safety Edge Sensor for Agv Automated Guided Vehicle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets.Best Design Felt Belt Type Cooling Table/Handling Extrusion System, 175-184[Essence Oil Amber Glass Bottle with Plastic Dropper]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling]

Whitaker,J.R.(1963),Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood. Analytical Chemistry35(12), 1950-1953[Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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