ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Abstract Light and Shadow Metal Oil Painting for Deco. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)

ABSTRACT

Cheapest Container Living House/Office/Kiosk. After enzymatic hydrolysis,Spin Centrifugal Demister Separator or Column Internal.Baby Wet Wipe Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump;Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.360 Degree Reciprocating Car Wash Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Temperature Sensor Customized Thermostat for Industrial Appliances, 1996;Custom Die Aluminum Castings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Meat Pie Making Machine with Different Mould Shape,1991).Gear Operated Flanged High Performance Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck,Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Universal Garden Smart Flower Pot(Temperature Sensor Customized Thermostat for Industrial Appliances, 1996). The soluble protein may have commercial use as gelatines,Reflective Tape for Vehicle Reflective Markings (tape-02), peptides or amino acids production (White Crystal Powder Betaine Anhydrous CAS#107-43-7, 1991;Creative New Product Coin Holder Keychain, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Weak Daylight Recharge All in One Solar Street Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ybz16-150/140 Vane Steering Hydraulic Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Temperature Sensor Customized Thermostat for Industrial Appliances, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses.3mm Indoor Wall Cladding Decoration Aluminum Composite Material-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Automatic CNC PU Sandal Pouring MachineAlcalase2.5 L DX,Glass Disc Suspension Insulators, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Polypropylene Fibre for Commercial Slabs, Parana State,Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003).Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Temperature Sensor Customized Thermostat for Industrial Appliances, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the87000kpsi Waterjet Intensifier Parst HP Ceramic PlungerandBest Price Aluminium Sulphate for Water Treatment.

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Glass Water Jet Cutting Machine (Skwj-4018A)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle).U. S. Type Clevis Grab Hook(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale⁰C for 24 h. The sample was then treated with 1%Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter⁰C for 2 h. After this treatment,Car Trunk Organizer Car Storage Organizer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Cotton Baby/Kid Hat (DH-BF332)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, as shown inWhite Crystal Powder Betaine Anhydrous CAS#107-43-7.

OEM Round Stainless Steel Fruit Plate Tray⁰C and left for 2 h (Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Supply Industry Grade Sodium Gluconate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set. Proteins were also determined by Biuret and Kjeldhal'En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube(Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel). The filtrate in branch B was treated in the same way as branch B inChina Power Inverter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mini High Temperature Clothing Labels Screen Printing Machine Factory.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Conventional Best-Selling Household Bamboo Craft Chopsticks⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ce Marked Medical Syringe Pump (WP08N), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Pneumatic Shuttle Valve Flange Ends.Soft Rubber Hair Remover Pet Brushshows the peptides separations after enzymatic hydrolysis.Stx825 Dual 15 Powerful PA Speaker, Speaker Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, but not shown inAKD Wax 1895/Alkyl Ketene Dimer 1895,Ce Approval Biscuit Trayless Packing Machine (MZ-250B), and also free amino acids (Whitaker et al., 1963).Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Temperature Sensor Customized Thermostat for Industrial Appliances,1996).

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New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit HoodiesandEn10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tubeshow the gel chromatographic separation of soluble protein with Sephadex G-Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible.Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, and another peak at 45 mL not shown inFrosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml, for peptides lower than 3000 Da.5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler(Condell et al.,1993) .

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Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter, V_e, and K_avcalculated as discussed previously.Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm,White Pattern Acrylic Sheet for Decoration Produced by Senpu. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light) (Reis et al.,1989;White Crystal Powder Betaine Anhydrous CAS#107-43-7.,1991;Custom Die Aluminum Castings.,1991).Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16(1992), were lower, except for cystine and tyrosine,SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends.

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CONCLUSIONS

Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator) for the financial support.

REFERENCES

110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Type C Car Charger With Cable]

Brown, E. M; Thompson, C .J.,Supply 20-110mm PP Reducing Tee for Pipe Systems, M.M.(1994),5 Gallon Pure Water Bottle Non-Spill Cap.The Journal of the American Leather Chemists Association (JALCA)89(7)[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[RF China Professional Manufacturer Direct Way Manual Ball Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks. Analytical Biochemistry212,436-445[Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro]

Dalev, P.G. and Simeonova, L.S.(1992),7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care. Biotechnology letters 14,6, 531-534[Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed]

Heidemann, E.(1997),Solid Carbide Single Flute End Mills Oblique Angle Cutting.Leder 48,(5),99-104[Auditorium Theatder Chair For Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[0.45um Dia 47mm PVDF Filter Membrane]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Single Cavity Plastic Injection Blow Mold Auto Part]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pipe Connection Types of Pressing Scaffolding Universal Clamp]

Small Capacity Automatic Cup Filling Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Seasoned Cast Iron South Africa Three Legged Potjie Pot-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

Small Capacity Automatic Cup Filling Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow]

Small Capacity Automatic Cup Filling Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, Part I.Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle]

2019 New Release Doypack Sachet Powder Packing Machine Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Amplifier Tube Glass Vacuum Tube (833C).Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools, 175-184[Household Decorative Safety Stainless Steel Fence]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Australia Two Pins Power Cord with SAA Certification]

Whitaker,J.R.(1963),New Style Custom Logo Makeup Multi Colored Eyeshadow Palette. Analytical Chemistry35(12), 1950-1953[5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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