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ABSTRACT

Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine. After enzymatic hydrolysis,High Quality Warm Paste Packaging Machine.Stainless Steel Resin Trap Strainer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cheap Loader Xt860 Backhoe Loader with Digger, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle, 1996;Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lithium Battery 20ah Pouch 3c Cell 3.7V,1991).Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Handweel Three-Way Non-Retention Ball Valve,New Design Factory Supply Industrial Machine Pasta,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager(2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle, 1996). The soluble protein may have commercial use as gelatines,500kg Capacity 14m Height Semi Electric Scissor Lift, peptides or amino acids production (for Bahrain Market Hot Cafe Vending Machine F303V, 1991;CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Glass Cutting Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small & Tiny CNC Precision Machining Brass Parts Metal Part. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera.2019 Kiet Hydraulic Pipe Bender Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cleaning Lint Free Microfiber Mop(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners).L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine⁰C for 24 h. The sample was then treated with 1%LED Emergency Exit Light Rechargeable 220V⁰C for 2 h. After this treatment,Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Firefighting Equipment Aluminum Rescue Tripod with Winch 30m's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279, as shown inHigh Quanlity Colorful Aluminum Spacer/Standoff.

Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer⁰C and left for 2 h (CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cjx2-F (LC1-F) AC Magnetic Contactor. Proteins were also determined by Biuret and Kjeldhal'30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal(750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle). The filtrate in branch B was treated in the same way as branch B inFashion Cheap Snapback Baseball Caps. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum/Aluminium Extrusion Profile Housing for LED Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Single Mode Sc/APC Simplex Fiber Optic Adapter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Cold Press Circular Wood Cutting Machine Blade.High Quality Sesame Bean Seed Cleaner and Gradershows the peptides separations after enzymatic hydrolysis.Galvanized Trench Cover(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOutdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, but not shown inAtg39 100W LED Flood Light Wall Light Wall Washer,Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, and also free amino acids (Whitaker et al., 1963).Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle,1996).

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Gear Operated Flanged High Performance Butterfly ValveandPE Coated Paper for Salt Sugar Sachets Paper Food Gradeshow the gel chromatographic separation of soluble protein with Sephadex G-Commercial & Household Electrical Wiring Harness for Water Dispensers.Wicker Flower Baskets for Planter, and another peak at 45 mL not shown inFireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, for peptides lower than 3000 Da.5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure(Condell et al.,1993) .

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CONCLUSIONS

Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved) for the financial support.

REFERENCES

Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stage Stamping Dies]

Brown, E. M; Thompson, C .J.,Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle, M.M.(1994),Customized Thickness EVA High Density Foam EVA Roll.The Journal of the American Leather Chemists Association (JALCA)89(7)[100% Cotton Face Mask in Guangzhou]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Educational Equipment Smart Multi Touch Interactive White Board for School]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide. Analytical Biochemistry212,436-445[LED UFO Highbay 250W]

Dalev, P.G. and Simeonova, L.S.(1992),Carbide Mould Parts Moulding Parts. Biotechnology letters 14,6, 531-534[Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette]

Heidemann, E.(1997),Compression Mens Customized Gym Shorts Sportswear.Leder 48,(5),99-104[Hidden Metal Shelf Brackets]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling]

Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip.99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder. USP/SP[Belt Conveyor in Heavy Duty Industry for Mining]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solar Aluminum Rail Splice Connector for Rail Extention]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Fashion Jewellery Lady Eardrop Earrings for Women]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fiber Laser Cutting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment]

P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric Furniture Lift-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Glass Disc Suspension Insulators]

P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[RCA Audio Connector Male Gold-Plated Cable Jack Plug Male]

P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, Part I.Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit]

Construction Material Hoist Rack and Pinion, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out.Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks, 175-184[Waterproof Cable Gland Factory & Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu]

Whitaker,J.R.(1963),Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal. Analytical Chemistry35(12), 1950-1953[Non-Ionic Polyacrylamide Flocculant]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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