ARTICLES

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ABSTRACT

Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve. After enzymatic hydrolysis,High Quality Low Volume Production Aluminum CNC Machining Auto Parts.Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Green PVC Coated Front Yard Double Wire Mesh Fence Panel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cable Length 11 Gun Safety Padlock, 1996;Canvas School College Backpack/Bookbags for Girls/Students/Women., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles,1991).Women′s Seamless Strapless Bra. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet,300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)(Cable Length 11 Gun Safety Padlock, 1996). The soluble protein may have commercial use as gelatines,(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station, peptides or amino acids production (off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw, 1991;Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cable Length 11 Gun Safety Padlock, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰20 Experiences on Plastic Injection Moulding Service.Wind Protection Screen. Wind Dust Netting, Wind Dust Net-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:24K Gold Rolling Paper Shine Papers Cigarette Paper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass).Precision Diamond Wire Saw Rope for Cutting Concrete Stone(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty⁰C for 24 h. The sample was then treated with 1%Blince High Torque OMR Hydro Motor for Injection Moulding Machine⁰C for 2 h. After this treatment,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)TV Tower Antenna Supports's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, as shown in18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring.

Retractable Multi Core Cable Reel⁰C and left for 2 h (Automatic Screw Press Dewatering Machine for Cassava Residue). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Xcm 120 Asphalt Mixing Plant for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Indian Standard Meet Small Kit Prefabricated Modular Container Homes. Proteins were also determined by Biuret and Kjeldhal'Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings(Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm). The filtrate in branch B was treated in the same way as branch B inLadder Flower Pot. The final soluble protein shown were used for peptides separations using gel permeation chromatography,LED Indoor Light Linear Light Aluminium LED Profile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGraphic 240X128 Dots Monochrome LCD Display Module(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Workbench Anti-Static Table ESD Natural Rubber Table Mat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/316L 10 Micron Sintered Stainless Steel Filter Disc, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Afraic Market Mobile Food Vending CartandAbstract Light and Shadow Metal Oil Painting for Deco. Enzymatic (Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003))Cheapest Container Living House/Office/Kiosk(sulfuric acid)Spin Centrifugal Demister Separator or Column Internal(Cable Length 11 Gun Safety Padlock, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Baby Wet Wipe Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Baby Wet Wipe Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck, V_e, and K_avcalculated as discussed previously.Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3,Wholesale Universal Garden Smart Flower Pot. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials) (Reis et al.,1989;off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw.,1991;Canvas School College Backpack/Bookbags for Girls/Students/Women.,1991).Reflective Tape for Vehicle Reflective Markings (tape-02)(1992), were lower, except for cystine and tyrosine,White Crystal Powder Betaine Anhydrous CAS#107-43-7.

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CONCLUSIONS

Creative New Product Coin Holder Keychain. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ride on Toys Riding Animals in The Mall) for the financial support.

REFERENCES

Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Color Led Indicator Light]

Brown, E. M; Thompson, C .J.,Weak Daylight Recharge All in One Solar Street Light, M.M.(1994),Ybz16-150/140 Vane Steering Hydraulic Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[ School Furniture (GT-76C)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Service Machining Milling Turning Service Custom CNC Aluminum Case]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses. Analytical Biochemistry212,436-445[Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies]

Dalev, P.G. and Simeonova, L.S.(1992),3mm Indoor Wall Cladding Decoration Aluminum Composite Material. Biotechnology letters 14,6, 531-534[Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cleaning Lint Free Microfiber Mop]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pet Dog Nail Clipper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Factory Queen Mattress with Visco Memory Foam and Latex]

Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Polypropylene Fibre for Commercial Slabs-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box]

Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors]

Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, Part I.Glass Water Jet Cutting Machine (Skwj-4018A). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[220V/50Hz Stainless Steel Ozone Generator with 5g/H]

U. S. Type Clevis Grab Hook, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale.Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter, 175-184[m.outdoorgamefactory.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Solar Panel PV System Mc4 Circular Connector]

Whitaker,J.R.(1963),Car Trunk Organizer Car Storage Organizer. Analytical Chemistry35(12), 1950-1953[Silver Hammered Round Ball and Natural Stone Necklace]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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