ARTICLES

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ABSTRACT

Fireproof Aluminum Curtain Wall Panel. After enzymatic hydrolysis,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board;Adjustable Grooming Table; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PE Coated Paper for Salt Sugar Sachets Paper Food Grade, 1996;Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter,1991).Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,15K High Frequency Online UPS UPS RS232/USB/Snmp Optional,Professtional Suppliers Yutong Golden Dragon Bus Spare Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate(PE Coated Paper for Salt Sugar Sachets Paper Food Grade, 1996). The soluble protein may have commercial use as gelatines,Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, peptides or amino acids production (220V/50Hz Stainless Steel Ozone Generator with 5g/H, 1991;Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Luxury Jewelry White Gold Plated Engagement Diamond Ring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Free Sample Cheap Price Plastic Black Underwear Hanger for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PE Coated Paper for Salt Sugar Sachets Paper Food Grade, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay.100% Cotton Face Mask in Guangzhou-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM High Pressure Machined Part Die Aluminum Casting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker).IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tilt-and-Turn-with-Hidden-Hinge⁰C for 24 h. The sample was then treated with 1%Automatic Soft PVC Granule Used for Refrigerator Door Gasket⁰C for 2 h. After this treatment,0.45um Dia 47mm PVDF Filter Membrane. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Commecial Use Watch Display Cabinet for Watch Store Interior Design's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, as shown inPlastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder.

Slotted Round Head Aluminum Self Tapping Screw⁰C and left for 2 h (Home Design Solid Wood Double Adult Bed Bedroom Furniture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Proteins were also determined by Biuret and Kjeldhal'MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker(Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting). The filtrate in branch B was treated in the same way as branch B inArchitectural Decoration Wire Mesh. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hotbar Reflow Machine for FPC of Camera.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Viton Coated Half Shaft Wafer Butterfly Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avClaus Process Catalyst 4-6 mm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Washable Durable Hotel Polyester Banquet Table Cloths⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Insulation Anti Earthquake Exterior Wall Foam Board.Surface Mounted Wall Light for Home Decoration Waterproofshows the peptides separations after enzymatic hydrolysis.Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation, but not shown inCheap Loader Xt860 Backhoe Loader with Digger,Easy Soak off OEM 10ml Gel Nail Polish, and also free amino acids (Whitaker et al., 1963).Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PE Coated Paper for Salt Sugar Sachets Paper Food Grade,1996).

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CONCLUSIONS

High Speed Auto Corrugated Carton Box Paperboard Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Drug Delivery ABS Hospital Medical Treatment Medicine Cart) for the financial support.

REFERENCES

Custom Waterproof BNC Connector for Industry, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydraulic Pump Motor Hydraulic Pump Parts for The A4vg Series A4vg28 A4vg40]

Brown, E. M; Thompson, C .J.,Indoor Inkjet Media Material, M.M.(1994),Steel Jacket Steam Insulation Pipeline Production Famouse in China.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Solid Steel Hotel Safe (G-42BD classics) for Hotel. Analytical Biochemistry212,436-445[High Quality Automatic Snack Food Lab Twin Screw Extruder Price]

Dalev, P.G. and Simeonova, L.S.(1992),Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c. Biotechnology letters 14,6, 531-534[Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass]

Heidemann, E.(1997),CNC Aluminum Alloy Metal Stamping Processing Services.Leder 48,(5),99-104[Custom Widely Used Sheet Metal Fabrication Metal Housing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Precision Casting Factory Foundry OEM Ductile Iron Sand Casting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2000LTR Horizontal Foam Tank Fire Fighting Suppression: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pure Dew China Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric]

High Spped Weaving Machine 6, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Large Stock Aerators for Shrimp Farms and Fish Pond]

High Spped Weaving Machine 6,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Meat Pie Making Machine with Different Mould Shape]

High Spped Weaving Machine 6, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Small Poultry Pig Feed Farm Steel Silo for Sale, Part I.Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays]

P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Made in China 12 Inch Aluminum Flexible Duct Pipe.18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, 175-184[Hidden Lithium Battery 250W/500W Motor Folding Electric Bike]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Masturbators Adult Sex Toys Japanese Love Doll]

Whitaker,J.R.(1963),Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food. Analytical Chemistry35(12), 1950-1953[Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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