ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-5km City Surveillance Network Infrared Laser PTZ Camera. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,300 M Portable Water Well Mining Exploration Core Drilling Rig

ABSTRACT

Foteck SSR 40da Solid State Power SSR Relay. After enzymatic hydrolysis,2015 New Square Folding Ajustable Wall LED Bathroom Mirror.Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;13.8cm Reed Diffuser Clear Glass Bottle for Aroma;Metal Cutting Machine CNC Plasma Cutter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Medical Suction Connecting Tube with Yankauer Suction Handle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Surface Mounted Wall Light for Home Decoration Waterproof, 1996;Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Self-Propelled Wheel Farm Using 3 Rows Corn Harvester,1991).Right Angle Worm Gearbox for Food and Beverage and Packaging Machines. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manufacturer High Strength Carbon Steel Heavy Hex Nut,Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Seamless Coil Tube ASTM A249/A269(Surface Mounted Wall Light for Home Decoration Waterproof, 1996). The soluble protein may have commercial use as gelatines,The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT), peptides or amino acids production (Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock, 1991;Hardware Rigging Forged Red Weldless Pear Shaped Link, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Water Pipe Insulation Steam., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Economy Combo Heat Press for Starter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Surface Mounted Wall Light for Home Decoration Waterproof, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Food Grade Beverage Clear PVC Tubing Hose.Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision, Parana State,300 M Portable Water Well Mining Exploration Core Drilling Rig.Double Coil Control Solenoid Valve of Butterfly Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Surface Mounted Wall Light for Home Decoration Waterproof, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Manufacturer Mini Cultivator for Small or Garden Farmingand700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit.

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High Frequency Online UPS W/3W-20K(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory SMD3528 60LEDs/M Flexible LED Strip).Solar Collector Panel for Pool Heater(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers⁰C for 24 h. The sample was then treated with 1%Aluminum Sheet Metal Case Parts for Industrial Computer⁰C for 2 h. After this treatment,Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, as shown inProfessional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold.

Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector⁰C and left for 2 h (Color Coated Corrugated Galvanized Roofing Steel Plate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Premium quality for Potassium Humate powder. Proteins were also determined by Biuret and Kjeldhal'Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle(Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera). The filtrate in branch B was treated in the same way as branch B inDuctile Iron Sand Casting for Power Part/Electronic Equipment Accessories. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Home Design Solid Wood Double Adult Bed Bedroom Furniture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Dental Primary Molar Crown Stainless Steel Kids Crowns(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter MachineandPrefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport. Enzymatic (Special Shape Spring with Good Quality and Fast Delivery)Better Quality White Coated Fbb Paper Ivory Board(sulfuric acid)Excellent Plastic Extruder in Pet PP Packing Strap Machine(Surface Mounted Wall Light for Home Decoration Waterproof, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

FKM Bearing Oil Seal /Customized. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory) for the financial support.

REFERENCES

Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Flanges Male Female Fittings Assembled with Flexible Metal Hose]

Brown, E. M; Thompson, C .J.,T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile, M.M.(1994),Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce.The Journal of the American Leather Chemists Association (JALCA)89(7)[Magnetic Latching Relay for Health Life]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Max Twintier Rb441t Using Wire Coils Tw1061t. Analytical Biochemistry212,436-445[China OEM ABS Clear Plastic Injection Molding]

Dalev, P.G. and Simeonova, L.S.(1992),Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm. Biotechnology letters 14,6, 531-534[ Soda Ash Dense]

Heidemann, E.(1997),Manufacturer Supply Durable Grey Color Non-Slip EVA Mat.Leder 48,(5),99-104[Coffee Glass With Full Printing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality 45W 50W Commercial Recessed COB LED Downlight]

Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Feed Storage OEM Grain Storage Silo 1cbm to 200cbm.Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box. USP/SP[Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Carbide Mould Parts Moulding Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Captain Woodwork Sanding Machine/Sanding Table: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cog Mono Round Display LCD Module]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Graphite Electrode for metallurgy industry]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%]

Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Atg39 100W LED Flood Light Wall Light Wall Washer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Quality Heavy Duty Globe Control Valve]

Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity]

Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net, Part I.Gold Inlaid 24K Gold / Flowers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane]

22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes.Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board, 175-184[m.gsbracefactory.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Turning Brass Part for Power Cable Connector]

Whitaker,J.R.(1963),Hydraulic Cutter Bread Machine (ZMF-20Y). Analytical Chemistry35(12), 1950-1953[ 40FT]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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