ARTICLES

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ABSTRACT

RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. After enzymatic hydrolysis,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.Cog Mono Round Display LCD Module. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black;460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Products Suppliers Camlock Coupling Quick Couplings Female, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, 1996;3.2m Vertical Wheelchair Elevator Platform for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LC Multistage Pump Mechanical Seal (KLLC),1991).Wedding Tent Cooling System Outdoor Event Air-Con Units. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV,Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prime Gi Steel Strip Galvanized Metal Strip Price(Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, 1996). The soluble protein may have commercial use as gelatines,Fireproof Aluminum Curtain Wall Panel, peptides or amino acids production (Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, 1991;High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Adjustable Grooming Table.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PE Coated Paper for Salt Sugar Sachets Paper Food Grade.Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High PerformanceAlcalase2.5 L DX,15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Professtional Suppliers Yutong Golden Dragon Bus Spare Parts); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, Parana State,Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947.220V/50Hz Stainless Steel Ozone Generator with 5g/H, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAISI 430 Ba/2b Embossed Stainless Steel SheetandAbstract Light and Shadow Metal Oil Painting for Deco.

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Corrugated Making Machinery

Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cute Cartoon Coin Purse Children Zipper Purse).Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Luxury Jewelry White Gold Plated Engagement Diamond Ring⁰C for 24 h. The sample was then treated with 1%Free Sample Cheap Price Plastic Black Underwear Hanger for Sale⁰C for 2 h. After this treatment,13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100% Cotton Face Mask in Guangzhou's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM High Pressure Machined Part Die Aluminum Casting, as shown inConstruction Material Hoist Rack and Pinion.

AISI 430 Ba/2b Embossed Stainless Steel Sheet⁰C and left for 2 h (China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Drug Delivery ABS Hospital Medical Treatment Medicine Cart. Proteins were also determined by Biuret and Kjeldhal'YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black(Sc Fiber Optic Fast Connector). The filtrate in branch B was treated in the same way as branch B inCnc Lathe Programming. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Colorful Electric English Funny Music Story Module Printing Book. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tilt-and-Turn-with-Hidden-Hinge⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Soft PVC Granule Used for Refrigerator Door Gasket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/0.45um Dia 47mm PVDF Filter Membrane, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Carbon Steel Spherical Plain Radical BearingsandCommecial Use Watch Display Cabinet for Watch Store Interior Design. Enzymatic (Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring)Slotted Round Head Aluminum Self Tapping Screw(sulfuric acid)Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike(Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Classic Black Color Commercial Glass Door Multi Deck Refrigerator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Classic Black Color Commercial Glass Door Multi Deck Refrigerator, flow rate of 0.1 mL/min., and l = 620 nm.

MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker. Carbon Steel 20g Gr. B Steam Cooling Fin Tubeshows the peptides separations after enzymatic hydrolysis.Hotbar Reflow Machine for FPC of Camera(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Factory Price Hg1 Self Adjustable Pipe Tank Rotator, but not shown inPuer/off White Melamine Particle Board/MDF Board,Full Viton Coated Half Shaft Wafer Butterfly Valve, and also free amino acids (Whitaker et al., 1963).Claus Process Catalyst 4-6 mm. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest,1996).

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Pickled Cucumber Production Line/Making MachineandDurable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooringshow the gel chromatographic separation of soluble protein with Sephadex G-4 Different Ball Silicone Ice Cube Maker.High Quality Washable Durable Hotel Polyester Banquet Table Cloths, and another peak at 45 mL not shown inHigh Quality Factory 8ml UV Spider Gel Nail Care, for peptides lower than 3000 Da.16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female(Condell et al.,1993) .

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Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, V_e, and K_avcalculated as discussed previously.200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision,Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic) (Reis et al.,1989;Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.,1991;3.2m Vertical Wheelchair Elevator Platform for Sale.,1991).Aerobic Fermentation Organic Fertilizer Agitator(1992), were lower, except for cystine and tyrosine,Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment.

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CONCLUSIONS

CS1363 a Standard UK 3 Pin Power Plug. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professtional Suppliers Yutong Golden Dragon Bus Spare Parts) for the financial support.

REFERENCES

Insulation Anti Earthquake Exterior Wall Foam Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Log Barker Debarker]

Brown, E. M; Thompson, C .J.,Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides, M.M.(1994),Easy Soak off OEM 10ml Gel Nail Polish.The Journal of the American Leather Chemists Association (JALCA)89(7)[ GRP Grating]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine. Analytical Biochemistry212,436-445[High Zinc Coated Galvanized Wire]

Dalev, P.G. and Simeonova, L.S.(1992),Recyclable PU Leather Jewelry Pouch. Biotechnology letters 14,6, 531-534[50kg Soya Bean Seed PP Woven Bags Sacks]

Heidemann, E.(1997),Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11).Leder 48,(5),99-104[Booster Pump Water Purifier Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)]

Customized UV Drying Heating Machine for Screen Printer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Activated Alumina Used for Catalyst Carrier in Chemical Industry.High Quality 45W 50W Commercial Recessed COB LED Downlight. USP/SP[Black Powder Coated Steel Sheet Metal Housing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[KZ-19/16/13 Pneumatic Combination Steel Binding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[50w Led Track Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Blue Silica Gel with Indicating]

1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)]

1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea]

1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, Part I.High Speed Auto Corrugated Carton Box Paperboard Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Conductor PE Insulated Triplex Cable]

Custom Waterproof BNC Connector for Industry, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Indoor Inkjet Media Material.Steel Jacket Steam Insulation Pipeline Production Famouse in China, 175-184[Automatic Pet Bottle Blow Molding Machine Blower]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment]

Whitaker,J.R.(1963),Solid Steel Hotel Safe (G-42BD classics) for Hotel. Analytical Chemistry35(12), 1950-1953[Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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